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초록· 키워드

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Wild-type V. vulnificus cannot grow using lactoseas the sole carbon source or take up the sugar. However,prolonged culture of this species in media containing lactose asthe sole carbon source leads to the generation of a spontaneouslactose-utilizing (LU) mutant. This mutant showed strong β-galactosidase activity, whereas the wild-type strain showed abarely detectable level of the activity. A mutant with a lesionin a gene homologous to the lacZ of E. coli in the bacteriumno longer showed β-galactosidase activity or generatedspontaneous LU mutants, sugesting that the lacZ homolog isresponsible for the catabolism of lactose, but the expresion ofthe gene and genes for transport of lactose is tightly regulated.Genetic analysis of spontaneous LU mutants showed that allthe mutations ocur in a lacI homolog, which is locateddownstream to the lacZ and putative ABC-type lac permeasegenes. Consistent with this, a genomic library clone containingthe lacI in trans, made the spontaneousLU mutants no longer able to utilize lactose as the sole carbonsource. Taken together with the observation that excesiveamounts of exogenously suplemented possible catabolic productsof lactose have negative effects on the growth and survivabilityof V. vulnificus, we sugest that V. vulnificus has evolved tocary a represor that tightly regulates the expression of lacZto keep the intracelular toxic catabolic intermediates at asublethal level.

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