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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제11호
- 발행연도
- 2011.1
- 수록면
- 1,151 - 1,158 (8page)
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In this study, a galactooligosaccharide (GOS) was synthesized using active β-galactosidase (β-gal) inclusion bodies (IBs)-containing Escherichia coli (E. coli) cells. Analysis by MALDI-TOF (matrix-assisted laser desorption/ionizationtime of flight) mass spectrometry revealed that a trisaccharide was the major constituent of the synthesized GOS mixture. Additionally, the optimal pH, lactose concentration, amounts of E. coli β-gal IBs, and temperature for GOS synthesis were 7.5, 500 g/l, 3.2 U/ml, and 37oC,respectively. The total GOS yield from 500 g/l of lactose under these optimal conditions was about 32%, which corresponded to 160.4 g/l of GOS. Western blot analyses revealed that β-gal IBs were gradually destroyed during the reaction. In addition, when both the reaction mixture and E. coli β-gal hydrolysate were analyzed by highperformance thin-layer chromatography (HP-TLC), the trisaccharide was determined to be galactosyl lactose,indicating that a galactose moiety was most likely transferred to a lactose molecule during GOS synthesis.
This GOS synthesis system might be useful for the synthesis of galactosylated drugs, which have recently received significant attention owing to the ability of the galactose molecules to improve the drugs solubility while decreasing their toxicity. β-Gal IB utilization is potentially a more convenient and economic approach to enzymatic GOS synthesis, since no enzyme purification steps after the transgalactosylation reaction would be required.
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참고문헌 (22)
참고문헌 신청
Ahmed, S. A / 2000 / Light chain of botulinum A neurotoxin expressed as an inclusion body from a synthetic gene is catalytically and functionally active / J. Protein Chem 19
Arie, J. P. / 2006 / Formation of active inclusion bodies in the periplasm of Escherichia coli / Mol. Microbiol 62 : 427 ~ 437
Botelho-Cunha, V. A / 2010 / Tailoring the enzymatic synthesis and nanofiltration fractionation of galacto-oligosaccharides / Biochem. Eng. J 50 : 29 ~ 36
Cardelle-Cobas, A / 2008 / Study of galacto-oligosaccharide formation from lactose using Pectinex Ultra SP-L / J. Sci. Food Agric 88 : 954 ~ 961
Crittenden, R. G / 1996 / Production, properties and applications of food-grade oligosaccharides / Trends Food Sci. Technol 7 : 353 ~ 361
Arie, J. P. / 2006 / Formation of active inclusion bodies in the periplasm of Escherichia coli / Mol. Microbiol 62 : 427 ~ 437
Botelho-Cunha, V. A / 2010 / Tailoring the enzymatic synthesis and nanofiltration fractionation of galacto-oligosaccharides / Biochem. Eng. J 50 : 29 ~ 36
Cardelle-Cobas, A / 2008 / Study of galacto-oligosaccharide formation from lactose using Pectinex Ultra SP-L / J. Sci. Food Agric 88 : 954 ~ 961
Crittenden, R. G / 1996 / Production, properties and applications of food-grade oligosaccharides / Trends Food Sci. Technol 7 : 353 ~ 361
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