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A response surface model was developed forpredicting the growth rates of Staphylococcus aureus intryptic soy broth (TSB) medium as a function of combineddiferent concentrations of NaCl (0, 2, 4, 6, 8, and 10%) wasadjusted to an initial of six diferent pH levels (pH 4, 5, 6, 7,8, 9, and 10) and incubated at 10, 20, 30, and 40oC. In alexperimental variables, the primary growth curves were well(r2=0.9000 to 0.9975) fitted to a Gompertz equation to obtaingrowth rates. The secondary response surface model fornatural logarithm transformations of growth rates as a functionof combined effects of temperature, pH, and NaCl was obtainedby SAS’s general linear analysis. The predicted growth ratesof the S. aureus were generally decreased by basic (pH 9-10) oracidic (pH 5-6) conditions and higher NaCl concentrations. Theresponse surface model was identified as an apropriate secondarymodel for growth rates on the basis of correlation coefficient(r=0.9703), determination coeficient (r2=0.9415), mean squareerror (MSE=0.0185), bias factor (Bf=1.0216), and accuracy factor(Af=1.2583). Therefore, the developed secondary model provedreliable for predictions of the combined effect of temperature,NaCl, and pH on growth rates for S. aureus in TSB medium.

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