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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제2호
- 발행연도
- 2013.1
- 수록면
- 246 - 250 (5page)
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초록· 키워드
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In this study, a loop-mediated isothermal amplification (LAMP) method to rapidly detect Staphylococcus aureus strains was developed and evaluated by extensively applying a large number of S. aureus isolates from clinical and food samples. Six primers were specially designed for recognizing eight distinct sequences on the species-specific femA gene of S. aureus. The detection limits were 100 fg DNA/tube and 104 CFU/ml. The LAMP assay was applied to 432 S. aureus strains isolated from 118 clinical and 314food samples. Total detection rates for the LAMP and polymerase chain reaction assays were 98.4% (306/311)and 89.4% (278/311), respectively.
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참고문헌 (21)
참고문헌 신청
Alarcon, B / 2006 / PCR-based procedures for detection and quantification of Staphylococcus aureus and their application in food / J. Appl. Microbiol 100 : 352 ~ 364
Goto, M / 2007 / Rapid detection of enterotoxigenic Staphylococcus aureus harbouring genes for four classical enterotoxins, SEA, SEB, SEC and SED, by loop-mediated isothermal
Hein, I / 2001 / Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of
Johnson, S / 1995 / FemA of Staphylococcus aureus: Isolation and immunodetection / FEMS. Microbiol. Lett 132 : 221 ~ 218
Margrit, S / 1990 / Temperature limits of growth, TNase and enterotoxin production of Staphylococcus aureus strains isolated from foods. Int / J. Food Microbiol 11 : 1 ~ 19
Goto, M / 2007 / Rapid detection of enterotoxigenic Staphylococcus aureus harbouring genes for four classical enterotoxins, SEA, SEB, SEC and SED, by loop-mediated isothermal
Hein, I / 2001 / Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of
Johnson, S / 1995 / FemA of Staphylococcus aureus: Isolation and immunodetection / FEMS. Microbiol. Lett 132 : 221 ~ 218
Margrit, S / 1990 / Temperature limits of growth, TNase and enterotoxin production of Staphylococcus aureus strains isolated from foods. Int / J. Food Microbiol 11 : 1 ~ 19
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