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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제11호
- 발행연도
- 2006.1
- 수록면
- 1,678 - 1,683 (6page)
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초록· 키워드
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A novel suspension-phase enzyme reaction systemL-ascorbic acidinto 2-O-α-D-glucopyranosyl L-ascorbic acid supplementingextrusion starch as the glycosyl donor was developed usingcyclodextrin glucanotransferase from Thermoanaerobactersp. A high conversion yield compared to the conventionalsoluble-phase enzyme reaction system using cyclodextrins andsoluble starch was achieved. The optimal reaction conditionswere 2,000 units of cyclodextrin glucanotransferase, 20 g/l ofLoC for24 h. The new suspension-phase enzyme reaction system alsoexhibited several distinct advantages other than a high conversionyield, including a lower acumulation of oligosacharides andeasily separable residual extrusion starch by centrifugation orfiltration in the reaction mixture, which will facilitate thepurification of 2-O-α-D-glucopyranosyl L-ascorbic acid. Thenew suspension-phase enzyme reaction system seems to bepotentially applicable as the industrial process for the productionO-α-D-glucopyranosylL-ascorbic acid.
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참고문헌 (21)
참고문헌 신청
Bae, K. M / 2001 / Purification and properties of cyclodextrin glucanotransferase synthesizing 2-O-α-D-glucopyranosyl L-ascorbic acid from Paenibacillus sp. JK-13 / J. Micro
Baek, S. G / 1993 / Reaction mechanism of transglycosylation of stevioside in the attrition coupled reaction system using raw starch as a glycosyl donor / Kor. J. Appl. Micro
Hajime, A / 1991 / Synthesis of 2-O-α-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus / Agric. Biol. Chem. 55 : 175
Horikoshi, K / 1999 / Alkaliphiles: Some applications of their products for biotechnology / Microbiol. Mol. Biol. Rev. 63 : 735 ~ 750
Jun, H. K / 1998 / Identification of L-ascorbic acid 2-O-α-glucoside, a stable form of ascorbic acid, in Kimchi / J. Microbiol. Biotechnol. 8 : 710 ~ 713
Baek, S. G / 1993 / Reaction mechanism of transglycosylation of stevioside in the attrition coupled reaction system using raw starch as a glycosyl donor / Kor. J. Appl. Micro
Hajime, A / 1991 / Synthesis of 2-O-α-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus / Agric. Biol. Chem. 55 : 175
Horikoshi, K / 1999 / Alkaliphiles: Some applications of their products for biotechnology / Microbiol. Mol. Biol. Rev. 63 : 735 ~ 750
Jun, H. K / 1998 / Identification of L-ascorbic acid 2-O-α-glucoside, a stable form of ascorbic acid, in Kimchi / J. Microbiol. Biotechnol. 8 : 710 ~ 713
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