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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제2호
- 발행연도
- 2017.1
- 수록면
- 289 - 296 (8page)
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초록· 키워드
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Lysine decarboxylase (CadA) converts L-lysine into cadaverine (1,5-pentanediamine), which is an important platform chemical with many industrial applications. Although there have been many efforts to produce cadaverine through the soluble CadA enzyme or Escherichia coli whole cells overexpressing the CadA enzyme, there have been few reports concerning the immobilization of the CadA enzyme. Here, we have prepared a cross-linked enzyme aggregate (CLEA) of E. coli CadA and performed bioconversion using CadACLEA. CadAfree and CadACLEA were characterized for their enzymatic properties. The optimum temperatures of CadAfree and CadACLEA were 60oC and 55oC, respectively. The thermostability of CadACLEA was significantly higher than that of CadAfree. The optimum pH of both enzymes was 6.0. CadAfree could not be recovered after use, whereas CadACLEA was rapidly recovered and the residual activity was 53% after the 10th recycle. These results demonstrate that CadACLEA can be used as a potential catalyst for efficient production of cadaverine.
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참고문헌 (23)
참고문헌 신청
Bhatia SK / 2015 / Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA / Bioprocess Biosyst. Eng 38 : 2315 ~ 23
Buschke N / 2011 / Metabolic engineering of Corynebacterium glutamicum for production of 1,5-diaminopentane from hemicellulose / Biotechnol. J 6 : 306 ~ 317
Cassan F / 2009 / Cadaverine production by Azospirillum brasilense and its possible role in plant growth promotion and osmotic stress mitigation / Eur. J. Soil Biol 45 : 12 ~
Chattopadhyay MK / 2003 / Polyamines protect Escherichia coli cells from the toxic effect of oxygen / Proc. Natl. Acad. Sci. USA 100 : 2261 ~ 2265
KANG, IN-HYE / 2007 / Cadaverine Protects Vibrio vulnificus from Superoxide Stress / Journal of Microbiology and Biotechnology 17 (1) : 176 ~ 179
Buschke N / 2011 / Metabolic engineering of Corynebacterium glutamicum for production of 1,5-diaminopentane from hemicellulose / Biotechnol. J 6 : 306 ~ 317
Cassan F / 2009 / Cadaverine production by Azospirillum brasilense and its possible role in plant growth promotion and osmotic stress mitigation / Eur. J. Soil Biol 45 : 12 ~
Chattopadhyay MK / 2003 / Polyamines protect Escherichia coli cells from the toxic effect of oxygen / Proc. Natl. Acad. Sci. USA 100 : 2261 ~ 2265
KANG, IN-HYE / 2007 / Cadaverine Protects Vibrio vulnificus from Superoxide Stress / Journal of Microbiology and Biotechnology 17 (1) : 176 ~ 179
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