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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제1호
- 발행연도
- 2017.1
- 수록면
- 77 - 83 (7page)
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Lignocellulosic biomass represents a potentially large resource to supply the world’s fuel and chemical feedstocks. Enzymatic bioconversion of this substrate offers a reliable strategy for accessing this material under mild reaction conditions. Owing to the complex nature of lignocellulose, many different enzymatic activities are required to function in concert to perform efficient transformation. In nature, large multienzyme complexes are known to effectively hydrolyze lignocellulose into constituent monomeric sugars. We created artificial complexes of enzymes, called rosettazymes, in order to hydrolyze glucuronoxylan, a common lignocellulose component, into its cognate sugar D-xylose and then further convert the D-xylose into D-xylonic acid, a Department of Energy top-30 platform chemical. Four different types of enzymes (endoxylanase, α-glucuronidase, β-xylosidase, and xylose dehydrogenase) were incorporated into the artificial complexes. We demonstrated that tethering our enzymes in a complex resulted in significantly more activity (up to 71%) than the same amount of enzymes free in solution. We also determined that varying the enzyme composition affected the level of complex-related activity enhancement as well as overall yield.
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참고문헌 (25)
참고문헌 신청
Balan V. / 2014 / Current challenges in commercially producing biofuels from lignocellulosic biomass / ISRN Biotechnol 2014 : 463074 ~
Bayer EA / 2004 / The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides / Annu. Rev. Microbiol 58 : 521 ~ 554
Borne R / 2013 / Unraveling enzyme discrimination during cellulosome assembly independent of cohesin-dockerin affinity / FEBS J 280 : 5764 ~ 5779
Chun BW / 2006 / The development of cement and concrete additive: based on xylonic acid derived via bioconversion of xylose / Appl. Biochem. Biotechnol 131 : 645 ~ 658
Dutta S / 2014 / Enzymatic breakdown of biomass:Enzyme active sites, immobilization, and biofuel production / Green Chem 16 : 4615 ~ 4626
Bayer EA / 2004 / The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides / Annu. Rev. Microbiol 58 : 521 ~ 554
Borne R / 2013 / Unraveling enzyme discrimination during cellulosome assembly independent of cohesin-dockerin affinity / FEBS J 280 : 5764 ~ 5779
Chun BW / 2006 / The development of cement and concrete additive: based on xylonic acid derived via bioconversion of xylose / Appl. Biochem. Biotechnol 131 : 645 ~ 658
Dutta S / 2014 / Enzymatic breakdown of biomass:Enzyme active sites, immobilization, and biofuel production / Green Chem 16 : 4615 ~ 4626
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