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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제12호
- 발행연도
- 2015.1
- 수록면
- 2,110 - 2,115 (6page)
이용수
초록· 키워드
오류제보하기
A liquid-based colorimetric assay using a pH indicator was introduced for high-throughput monitoring of lysine decarboxylase activity. The assay is based on the color change of bromocresol purple, measured at 595 nm in liquid reaction mixture, due to an increase of pH by the production of cadaverine. Bromocresol purple was selected as the indicator because it has higher sensitivity than bromothymol blue and pheonol red within a broad range and shows good linearity within the applied pH. We applied this for simple determination of lysine decarboxylase reusability using 96-well plates, and optimization of conditions for enzyme overexpression with different concentrations of IPTG on lysine decarboxylase. This assay is expected to be applied for monitoring and quantifying the liquid-based enzyme reaction in biotransformation of decarboxylase in a high-throughput way.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Banerjee A / 2003 / A highthroughput amenable colorimetric assay for enantioselective screening of nitrilase-producing microorganisms using pH sensitive indicators / J. Biomo
Bhatia SK / 2015 / Starch-based polyhydroxybutyrate production in engineered Escherichia coli / Bioprocess Biosyst Eng 38 : 1479 ~ 1484
Bover-Cid S / 1999 / Improved screening procedure for biogenic amine production by lactic acid bacteria / Int. J. Food Microbiol 53 : 33 ~ 41
Brooker DC / 1973 / Rapid test for lysine decarboxylase activity in Enterobacteriaceae / Appl. Microbiol 26 : 622 ~ 623
Flores HE / 1982 / Analysis of polyamines in higher plants by high performance liquid chromatography / Plant Physiol 69 : 701 ~ 706
Bhatia SK / 2015 / Starch-based polyhydroxybutyrate production in engineered Escherichia coli / Bioprocess Biosyst Eng 38 : 1479 ~ 1484
Bover-Cid S / 1999 / Improved screening procedure for biogenic amine production by lactic acid bacteria / Int. J. Food Microbiol 53 : 33 ~ 41
Brooker DC / 1973 / Rapid test for lysine decarboxylase activity in Enterobacteriaceae / Appl. Microbiol 26 : 622 ~ 623
Flores HE / 1982 / Analysis of polyamines in higher plants by high performance liquid chromatography / Plant Physiol 69 : 701 ~ 706
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