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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제4호
- 발행연도
- 2008.1
- 수록면
- 754 - 758 (5page)
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초록· 키워드
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Agrobacterium tumefaciens-mediated transformation (ATMT) was successfully applied to Monascus ruber. The optimum cocultivation time was 84 h with an efficiency of 900 to 1,000 transformants when 1×106 spores were used with the same volume of bacteria. The stability of transformants was over 98% after five generations. When M. ruber was transformed with A. tumefaciens YL-63 containing the green fluorescent protein gene (egfp), the green fluorescent signal was observed throughout hyphae, confirming expression of the gene. This efficient transformation and expression system of M. ruber by ATMT will facilitate the study of this fungus at a molecular genetic level.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Blanc, P. J. / 1995 / Characterization of monascidin A from Monascus as citrinin.Int.J.Food Microbiol.27:201-213 / Food Microbiol 27 : 201 ~ 213
Blanc, P. J. / 1995 / Production of citrinin by various species of Monascus. Biotechnol / Lett 17 : 291 ~ 294
Bundock, P. / 1995 / Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae / EMBO 14 : 3206 ~ 3214
Campoy, S. / 2003 / Stable transformants of the azaphilone pigment-producing Monascus purpureus obtained by protoplast transformation and Agrobacterium-mediated DNA transfer.
Endo, A. / 1979 / Monacolin K, a new hypocholesterolemic agent produced by a Monascus species / Antibiot 32 : 852 ~ 854
Blanc, P. J. / 1995 / Production of citrinin by various species of Monascus. Biotechnol / Lett 17 : 291 ~ 294
Bundock, P. / 1995 / Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae / EMBO 14 : 3206 ~ 3214
Campoy, S. / 2003 / Stable transformants of the azaphilone pigment-producing Monascus purpureus obtained by protoplast transformation and Agrobacterium-mediated DNA transfer.
Endo, A. / 1979 / Monacolin K, a new hypocholesterolemic agent produced by a Monascus species / Antibiot 32 : 852 ~ 854
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