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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제2호
- 발행연도
- 2019.1
- 수록면
- 230 - 234 (5page)
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초록· 키워드
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Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplastmediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.
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참고문헌 (18)
참고문헌 신청
Yamada R / 2014 / Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose / Microb. Cell Fact 13:71
Kitamoto K / 2015 / Cell biology of the Koji mold Aspergillus oryzae / Biosci. Biotechnol. Biochem 79:863~869
Wang B / 2010 / Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing / Nucleic. Acids Res 38:5075~5087
Ichishima E / 2016 / Development of enzyme technology for Aspergillus oryzae, A, sojae, and A. luchuensis, the national microorganisms of Japan / Biosci. Biotechnol. Biochem 80:1681~1692
Suzuki S / 2009 / A novel transformation system using a bleomycin resistance marker with chemosensitizers for Aspergillus oryzae / Biochem. Biophys. Res. Commun 383:42~47
Kitamoto K / 2015 / Cell biology of the Koji mold Aspergillus oryzae / Biosci. Biotechnol. Biochem 79:863~869
Wang B / 2010 / Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing / Nucleic. Acids Res 38:5075~5087
Ichishima E / 2016 / Development of enzyme technology for Aspergillus oryzae, A, sojae, and A. luchuensis, the national microorganisms of Japan / Biosci. Biotechnol. Biochem 80:1681~1692
Suzuki S / 2009 / A novel transformation system using a bleomycin resistance marker with chemosensitizers for Aspergillus oryzae / Biochem. Biophys. Res. Commun 383:42~47
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