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- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제1호
- 발행연도
- 2011.1
- 수록면
- 37 - 42 (6page)
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A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was optimized for quantification of acrylic acid,acrylamide, and acrylonitrile. The optimal medium conditions for nitrilase activity were pH of 7.0, temperature of 30oC,agitation of 150 rpm, and inoculum level of 2%. Glycerol as a carbon source and sodium nitrate as the nitrogen source provided good nutritional sources for achieving good biotransformation. Nitrilase activity was constitutive in nature and was in the exponential growth phase after 24 h of incubation under optimal conditions without addition of any inducer. The substrate preference was acrylonitrile and acetonitrile. The present work demonstrates the biotransformation of acrylonitrile to acrylic acid with the new strain, R. ruber AKSH-84, which can be used in green biosynthesis of acrylic acid for biotechnological processes.
The nitrilase produced by the isolate was purified and characterized.
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참고문헌 (23)
참고문헌 신청
Alcalde, M / 2006 / Environmental biocatalysis: From remediation with enzymes to novel green processes / Trends Biotechnol 24 : 281 ~ 287
American Public Health Association / 1992 / Standard Methods for the Examination of Water and Waste Water, 18th Ed / American Public Health Association, American Water Works Association
Andhale, M. S / 1996 / Isolation and screening of acrylamide-producing microorganisms.Indian J.Exp.Biol.34:1005-1009 / Indian J. Exp. Biol 34 : 1005 ~ 1009
Anonymous / / Acrylic acid: 2010 World market outlook and forecast
Anonymous / / PERP Program - Acrylic acid. Report No. 04/05-6
American Public Health Association / 1992 / Standard Methods for the Examination of Water and Waste Water, 18th Ed / American Public Health Association, American Water Works Association
Andhale, M. S / 1996 / Isolation and screening of acrylamide-producing microorganisms.Indian J.Exp.Biol.34:1005-1009 / Indian J. Exp. Biol 34 : 1005 ~ 1009
Anonymous / / Acrylic acid: 2010 World market outlook and forecast
Anonymous / / PERP Program - Acrylic acid. Report No. 04/05-6
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