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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제2호
- 발행연도
- 2008.1
- 수록면
- 234 - 241 (8page)
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초록· 키워드
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Agrobacterium tumefaciens-mediated transformation (ATMT) is becoming an effective system as an insertional mutagenesis tool in filamentous fungi. We developed and optimized ATMT for two Colletotrichum species, C. falcatum and C.
acutatum, which are the causal agents of sugarcane red rot and pepper anthracnose, respectively. A. tumefaciens strain SK1044, carrying a hygromycin phosphotransferase gene (hph) and a green fluorescent protein (GFP) gene, was used to transform the conidia of these two Colletotrichum species. Transformation efficiency was correlated with cocultivation time and bacterial cell concentration and was higher in C. falcatum than in C. acutatum. Southern blot analysis indicated that about 65% of the transformants had a single copy of the T-DNA in both C. falcatum and C.
acutatum and that T-DNA integrated randomly in both fungal genomes. T-DNA insertions were identified in transformants through thermal asymmetrical interlaced PCR (TAIL-PCR) followed by sequencing. Our results suggested that ATMT can be used as a molecular tool to identify and characterize pathogenicity-related genes in these two economically important Colletotrichum species.
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
[학술지(정기간행물
Abuodeh, R. O. / 2000 / Genetic transformation of Coccidioides immitis facilitated by Agrobacterium tumefaciens. J. Infect / Dis 181 : 2106 ~ 2110
Alexander, K. / 1992 / Current status of red rot disease of sugarcane in Tamil Nadu. South Indian Sugarcane Sugarcane Technol / Assoc : 61 ~ 65
Balhadere, P. V. / 1999 / Identification of pathogenicity mutants of the rice blast fungus Magnaporthe grisea by insertional mutagenesis. Mol / Plant- Microbe Interact 12 : 1
Bundock, P. / 1995 / Transkingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae / EMBO 14 : 3206 ~ 3214
Abuodeh, R. O. / 2000 / Genetic transformation of Coccidioides immitis facilitated by Agrobacterium tumefaciens. J. Infect / Dis 181 : 2106 ~ 2110
Alexander, K. / 1992 / Current status of red rot disease of sugarcane in Tamil Nadu. South Indian Sugarcane Sugarcane Technol / Assoc : 61 ~ 65
Balhadere, P. V. / 1999 / Identification of pathogenicity mutants of the rice blast fungus Magnaporthe grisea by insertional mutagenesis. Mol / Plant- Microbe Interact 12 : 1
Bundock, P. / 1995 / Transkingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae / EMBO 14 : 3206 ~ 3214
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