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In order to detect chitooligosaccharides (COS) insoybean paste, tandem immunoaffinity chromatography andenzyme-linked immunosorbent assay (ELISA) were developed.Polyclonal anti-chitooligosaccharides mixture (COSM) antibodyspecific to COSM was attached to Sepharose gel for initialsample cleanup and concentration of COS in soybean paste.COS was eluted and quantified by competitive direct ELISA(cdELISA). Average ELISA recoveries from the columnusing binding buffer spiked with COSM at levels of 0.5, 2.0,5.0, and 10.0 mg/ml were 79.8, 72.0, 77.7, and 60.6%,respectively, with a mean recovery of 72.5%. Mean inter-welland inter-assay coefficients of variation (CV) were 7.7% and10.3%, respectively. Average recoveries from soybean pastespiked with COSM at levels of 2, 6, 20, and 60 mg/g were115, 91.7, 91, and 73.3%, respectively, with a mean recoveryof 92.8%. Mean inter-well and inter-assay CV were 12.9%and 16%, respectively. The COS was detected from 24 out of25 homemade Korean soybean paste samples at an average of14.0 mg/g (n, 25; range, 0- 51.2 mg/g) and from 13 out of 14commercially made soybean paste samples at an average of4.1 mg/g (n, 14; range, 0- 18.4 mg/g). The tandem immunoaffinitychromatography-cdELISA that was developed in this studyshowed that the level of COS eluted from homemade soybeanpaste was higher than that of the commercially made ones. Inaddition, the level of COS eluted from commercially availablesoybean paste in Korea was higher than that of the ones inJapan.

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