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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제2호
- 발행연도
- 2005.1
- 수록면
- 330 - 336 (7page)
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초록· 키워드
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Lactococcus lactis A164 is a nisin Z-producingstrain isolated from kimchi. Its antimicrobial spectrum hasbeen found to be active against most Gram-positive bacteriatested, yet inactive against Gram-negative bacteria [3].Accordingly, to overcome this drawback, the current studyattempted to express human β-defensin-1 (hBD-1), which killsboth Gram-positive and Gram-negative bacteria in L. lactisA164. When the hBD-1 cDNA was introduced using a nisinZ-controlled expression cassette, the L. lactis A164 transformantsgrew very poorly, due to the bactericidal effect of the expressedhBD-1 against the transformants. Therefore, a gene fusionsystem was designed to reduce the toxicity of the expressedheterologous protein against the host cells. As such, thehBD-1 gene was fused to the DsbC-Tag of pET-40b(+), thenintroduced to L. lactis A164. The transformants expressedan intracellular 35.6-kDa DsbC-hBD-1 fusion protein thatexhibited slight activity against the host cells, yet not enoughto strongly inhibit the cell growth. To obtain the recombinanthBD-1, the DsbC-hBD-1 fusion protein was purified bynickel-affinity column chromatography, and the DsbC-Tagremoved by cleaving with enterokinase. The cleaved maturehBD-1 exhibited strong bactericidal activity against E. coliJM109, indicating that the recombinant L. lactis A164 produceda biologically active hBD-1. In addition, the recombinant L.lactis A164 was also found to produce the same level of nisinZ as the wild-type.
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참고문헌 (29)
참고문헌 신청
-A / 2000 / Expression of Staphylococcus aureus clumping factorA in Lactococcus lactis subsp 68 : 3516 ~ 3522
/ 1993 / Genetics of bacteriocins producedby lactic acid bacteria : 39 ~ 85
Anderson / 1983 / Simple andrapid method for isolating large plasmid DNA from lacticstreptococci : 549 ~ 552
Choi / 2000 / Production of a nisin-like bacteriocin by Lactococcus lactisFig Comparison of cell growth patterns of L in M17 broth at 30oC lactis A164 isolated from kimchi :
Ganz / 1999 / Defensins and host defense 286 : 420 ~ 421
/ 1993 / Genetics of bacteriocins producedby lactic acid bacteria : 39 ~ 85
Anderson / 1983 / Simple andrapid method for isolating large plasmid DNA from lacticstreptococci : 549 ~ 552
Choi / 2000 / Production of a nisin-like bacteriocin by Lactococcus lactisFig Comparison of cell growth patterns of L in M17 broth at 30oC lactis A164 isolated from kimchi :
Ganz / 1999 / Defensins and host defense 286 : 420 ~ 421
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