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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제12호
- 발행연도
- 2009.1
- 수록면
- 1,695 - 1,702 (8page)
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초록· 키워드
오류제보하기
An animal-component-free and chemically defined fedbatch
process for GS-engineered cell lines producing
recombinant antibodies has been developed. The fedbatch
process relied on supplying sufficient nutrients to
match their consumption, simultaneously minimizing the
accumulation of by-products (lactate and osmolality). The
proportionalities of nutritional consumption were determined
by direct analysis. The robust, metabolically responsive
feeding strategy was based on the offline measurement of
glucose. The fed-batch process was shown to perform
equivalently in GS-CHO and GS-NS0 cultures. Compared
with batch cultures, the fed-batch technology generated
the greater increase in cell yields (5-fold) and final
antibody concentrations (4-8-fold). The majority of the
increase in final antibody concentration was a function of
the increased cell density and the prolonged culture time.
This generic and high-yielding fed-batch process would
shorten development time, and ensure process stability,
thereby facilitating the manufacture of therapeutic antibodies
by GS-engineered cell lines.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Barnes, L. M. / 2000 / Advances in animal cell recombinant protein production: GSNS0 expression system / Cytotechnology 32 : 109 ~ 123
Bibila, T. A. / 1995 / In pursuit of the optimal fed-batch process for monoclonal antibody production / Biotechnol. Prog 11 : 1 ~ 13
Birch, J. R. / 2006 / Antibody production / Adv. Drug Deliver. Rev 58 : 671 ~ 685
Brown, M. E. / 1992 / Process development for the production of recombinant antibodies using the glutamine synthetase (GS) system / Cytotechnology 9 : 231 ~ 236
Burky, J. E. / 2007 / Protein-free fed-batch culture of non-GS NS0 cell lines for production of recombinant antibodies / Biotechnol. Bioeng 96 : 281 ~ 293
Bibila, T. A. / 1995 / In pursuit of the optimal fed-batch process for monoclonal antibody production / Biotechnol. Prog 11 : 1 ~ 13
Birch, J. R. / 2006 / Antibody production / Adv. Drug Deliver. Rev 58 : 671 ~ 685
Brown, M. E. / 1992 / Process development for the production of recombinant antibodies using the glutamine synthetase (GS) system / Cytotechnology 9 : 231 ~ 236
Burky, J. E. / 2007 / Protein-free fed-batch culture of non-GS NS0 cell lines for production of recombinant antibodies / Biotechnol. Bioeng 96 : 281 ~ 293
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