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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제1호
- 발행연도
- 2014.1
- 수록면
- 59 - 69 (11page)
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One of the major challenges in metabolic engineering for enhanced synthesis of value-addedchemicals is to design and develop new strains that can be translated into well-controlledfermentation processes using bioreactors. The aim of this study was to assess the influence ofvarious fed-batch strategies in the performance of metabolically engineered Pseudomonasputida strains, Δgcd and Δgcd-pgl, for improving production of medium-chain-length polyhydroxyalkanoates(mcl-PHAs) using glucose as the only carbon source. First we developed afed-batch process that comprised an initial phase of biomass accumulation based on anexponential feeding carbon-limited strategy. For the mcl-PHA accumulation stage, threeinduction techniques were tested under nitrogen limitation. The substrate-pulse feeding wasmore efficient than the constant-feeding approach to promote the accumulation of thedesirable product. Nonetheless, the most efficient approach for maximum PHA synthesis wasthe application of a dissolved-oxygen-stat feeding strategy (DO-stat), where P. putida Δgcdmutant strain showed a final PHA content and specific PHA productivity of 67% and0.83 g·l-1·h-1, respectively. To our knowledge, this mcl-PHA titer is the highest value that hasbeen ever reported using glucose as the sole carbon and energy source. Our results alsohighlighted the effect of different fed-batch strategies upon the extent of realization of theintended metabolic modification of the mutant strains.
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참고문헌 (21)
참고문헌 신청
Chen G-Q. / 2009 / A microbial polyhydroxyalkanoates (PHA)based bio- and materials industry / Chem. Soc. Rev 38 : 2434 ~ 2446
Diniz S / 2004 / High-cell-density cultivation of Pseudomonas putida IPT 046 and medium-chain-length polyhydroxyalkanoate production from sugarcane carbohydrates / Appl. Bioc
Escapa I / 2011 / Disruption of B-oxidation pathway in Pseudomonas putida KT2442 to produce new functionalized PHAs with thioester groups / Appl. Microbiol. Biotechnol 89 : 1
Follonier S / 2011 / A reduction in growth rate of Pseudomonas putida KT2442 counteracts productivity advances in medium-chain-length polyhydroxyalkanoate production from glu
Frazzetto G. / 2003 / White biotechnology / EMBO Rep 4 : 835 ~ 837
Diniz S / 2004 / High-cell-density cultivation of Pseudomonas putida IPT 046 and medium-chain-length polyhydroxyalkanoate production from sugarcane carbohydrates / Appl. Bioc
Escapa I / 2011 / Disruption of B-oxidation pathway in Pseudomonas putida KT2442 to produce new functionalized PHAs with thioester groups / Appl. Microbiol. Biotechnol 89 : 1
Follonier S / 2011 / A reduction in growth rate of Pseudomonas putida KT2442 counteracts productivity advances in medium-chain-length polyhydroxyalkanoate production from glu
Frazzetto G. / 2003 / White biotechnology / EMBO Rep 4 : 835 ~ 837
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