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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제9호
- 발행연도
- 2013.1
- 수록면
- 1,327 - 1,338 (12page)
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The humanized anti-hepatocyte growth factor (HGF) monoclonal antibody (mAb) YYB-101 is a promising therapeutic candidate for treating various cancers. In this study, we developed a bioprocess for large-scale production of YYB-101 and evaluated its therapeutic potential for tumor treatment using a xenograft mouse model. By screening diverse chemically defined basal media formulations and by assessing the effects of various feed supplements and feeding schedules on cell growth and antibody production, we established an optimal medium and feeding method to produce 757 mg/l of YYB-101 in flask cultures, representing a 7.5-fold increase in titer compared with that obtained under non-optimized conditions. The optimal dissolved oxygen concentration for antibody production was 70% pO2. A pH shift from 7.2 to 7.0, rather than controlled pH of either 7.0 or 7.2, resulted in productivity improvement in 5 L and 200 L bioreactors, yielding 737 and 830 mg/ml of YYB-101, respectively. The YYB-101mAb highly purified by affinity chromatography using a Protein A column and two-step ion exchange chromatography effectively neutralized HGF in a cell-based assay and showed potent tumor suppression activity in a mouse xenograft model established with human glioblastoma cells.
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참고문헌 (21)
참고문헌 신청
Borset M / 1996 / Hepatocyte growth factor reverses the TGF-beta-induced growth inhibition of CCL-64 cells. A novel bioassay for HGF and implications for the TGF-beta bioassa
Bottaro DP / 1991 / Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product / Science 251 : 802 ~ 804
Burgess T / 2006 / Fully human monoclonal antibodies to hepatocyte growth factor with therapeutic potential against hepatocyte growth factor/c-Met-dependent human tumors / Ca
Butler M. / 2005 / Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals / Appl. Microbiol. Biotechnol 68 : 283 ~ 291
Cao B / 2001 / Neutralizing monoclonal antibodies to hepatocyte growth factor/scatter factor (HGF/SF) display antitumor activity in animal models / Proc. Natl. Acad. Sci. USA 98 :
Bottaro DP / 1991 / Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product / Science 251 : 802 ~ 804
Burgess T / 2006 / Fully human monoclonal antibodies to hepatocyte growth factor with therapeutic potential against hepatocyte growth factor/c-Met-dependent human tumors / Ca
Butler M. / 2005 / Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals / Appl. Microbiol. Biotechnol 68 : 283 ~ 291
Cao B / 2001 / Neutralizing monoclonal antibodies to hepatocyte growth factor/scatter factor (HGF/SF) display antitumor activity in animal models / Proc. Natl. Acad. Sci. USA 98 :
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