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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제7호
- 발행연도
- 2008.1
- 수록면
- 1,216 - 1,220 (5page)
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초록· 키워드
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Sequence analysis of the metabolically rich genome of
Streptomyces peucetius ATCC 27952 revealed a 2,199 bp
sesquiterpene alcohol (germacradienol) synthase-encoding
gene from the germacradienol synthase/terpene cyclase
gene cluster. The gene was named spterp13, and its
putative function is as a germacradienol synthase/terpene
cyclase. The amino acid sequence of Spterp13 shows 66%
identity with SAV2163 (GeoA) from S. avermitilis MA-
4680 and 65% identity with SCO6073 from S. coelicolor
A3(2), which produces germacradienol/geosmin. The fulllength
recombinant protein was heterologously expressed
as a his-tagged fusion protein in Escherichia coli, purified,
and shown to catalyze the Mg2+-dependent conversion of
farnesyl diphosphate to the germacradienol, which was
verified by gas chromatography/mass spectrometry.
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