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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제8호
- 발행연도
- 2018.1
- 수록면
- 1,293 - 1,298 (6page)
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Phosphomannomutase (ManB) converts mannose-6-phosphate (M-6-P) to mannose-1- phosphate (M-1-P), which is a key metabolic precursor for the production of GDP-D-mannose used for production of glycoconjugates and post-translational modification of proteins. The aim of this study was to express the manB gene from Escherichia coli in Lactococcus lactis subsp. cremoris NZ9000 and to characterize the encoded enzyme. The manB gene from E. coli K12, of 1,371 bp and encoding 457 amino acids (52 kDa), was cloned and overexpressed in L. lactis NZ9000 using the nisin-controlled expression system. The enzyme was purified by Ni-NTA column chromatography and exhibited a specific activity of 5.34 units/mg, significantly higher than that of other previously reported ManB enzymes. The pH and temperature optima were 8.0 and 50°C, respectively. Interestingly, the ManB used in this study had two substrate specificity for both mannose-1-phosphate and glucose-1-phosphate, and the specific activity for glucose-1-phosphate was 3.76 units/mg showing 70% relative activity to that of mannose- 1-phosphate. This is the first study on heterologous expression and characterization of ManB in lactic acid bacteria. The ManB expression system constructed in this study canbe used to synthesize rare sugars or glycoconjugates.
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참고문헌 (21)
참고문헌 신청
Leroy F / 2004 / Lactic acid bacteria as functional starter cultures for the food fermentation industry / Food Sci. Technol. 15:67~78
Ouwehand AC / 2002 / Probiotics: an overview of beneficial effects / Antonie Van Leeuwenhoek 82:279~289
Peterbauer C / 2011 / Food-grade gene expression in lactic acid bacteria / Biotechnol. J. 6:1147~1161
Mierau I / 2005 / 10 years of the nisincontrolled gene expression system (NICE) in Lactococcus lactis / Appl. Microbiol. Biotechnol. 68:705~717
Li L / 2018 / Emerging Areas in Bioengineering: Application of lactic acid bacteria for food biotechnology / Wiley-VCH :375~398
Ouwehand AC / 2002 / Probiotics: an overview of beneficial effects / Antonie Van Leeuwenhoek 82:279~289
Peterbauer C / 2011 / Food-grade gene expression in lactic acid bacteria / Biotechnol. J. 6:1147~1161
Mierau I / 2005 / 10 years of the nisincontrolled gene expression system (NICE) in Lactococcus lactis / Appl. Microbiol. Biotechnol. 68:705~717
Li L / 2018 / Emerging Areas in Bioengineering: Application of lactic acid bacteria for food biotechnology / Wiley-VCH :375~398
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