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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 136 - 143 (8page)
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초록· 키워드
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DNA fragments encoding the ketosynthase (KS) domain of polyketide synthase (PKS) genes were amplified using polymerase chain reaction (PCR) from 9 strains of Sorangium cellulosum isolated in Korea, cloned into a plasmid vector and sequenced. A total of 83 cloned DNA fragments were analyzed, and similar fragments were excluded, leaving 43 independent DNA fragments encoding the KS domains. The predicted amino acid sequences of 32 fragments were 70%-100% identical to the amino acid sequences of already known PKS genes, while the remaining 11 fragments were ≤67% or less identical to the known sequences, suggesting that these genes are novel PKS genes.
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참고문헌 (25)
참고문헌 신청
Bode, H. B / 2006 / Analysis of myxobacterial secondary metabolism goes molecular / J. Ind. Microbiol. Biotechnol. 33 : 577 ~ 588
Cane, D. E / 1998 / Harnessing the biosynthetic code: combinations, permutations, and mutations / Science 282 : 63 ~ 68
Finking, R / 2004 / Biosynthesis of nonribosomal peptides / Annu. Rev. Microbiol. 58 : 453 ~ 488
Frank, B / 2007 / Spiroketal polyketide formation in Sorangium: identification and analysis of the biosynthetic ene cluster for the highly cytotoxic spirangienes / Chem. Biol
Gerth, K / 2003 / Myxobacteria: proficient producers of novel natural products with various biological activities-past and future biotechnological aspects with the focus on t
Cane, D. E / 1998 / Harnessing the biosynthetic code: combinations, permutations, and mutations / Science 282 : 63 ~ 68
Finking, R / 2004 / Biosynthesis of nonribosomal peptides / Annu. Rev. Microbiol. 58 : 453 ~ 488
Frank, B / 2007 / Spiroketal polyketide formation in Sorangium: identification and analysis of the biosynthetic ene cluster for the highly cytotoxic spirangienes / Chem. Biol
Gerth, K / 2003 / Myxobacteria: proficient producers of novel natural products with various biological activities-past and future biotechnological aspects with the focus on t
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Expression of DNA-dependent Protein Kinase and Its Relationship with Epidermal Growth Factor Receptor Signaling in Metastatic Cancer Cell Lines
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Computational Approach for Biosynthetic Engineering of Post-PKS Tailoring Enzymes
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Combinatorial Biosynthesis of Polyketides : Generation of Multiple Bioactive Macrolides by Hybrid Polyketide Synthases
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Computational System for the Analysis of Post-PKS Glycosylation Step
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Soraphen 생합성 유전자군을 갖는 점액세균의 분리
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Isolation of Sorangium cellulosum Carrying Epothilone Gene Clusters
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Polyketide 이차대사물질의 생합성
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Enhanced Production of Epothilone by Immobilized Sorangium cellulosum in Porous Ceramics
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Sequence-Based Screening for Putative Polyketide Synthase Gene-Harboring Clones from a Soil Metagenome Library
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Self-reciprocal polynomials with related maximal zeros
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