지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Fibrin clots of blood vessels are one of the serious factor caused cardiovascular disease. The development of a antithrombotic and thrombolysis solvent is necessary to prevent and treat these diseases. It has been reported that a strong fibrin-specific fibrinolytic enzyme was produced from a Korean fermented soybean paste similar to Japanese miso. We have been screened the known or novel fibrinolytic enzymes by activity-based and sequence-based screening from soil DNA metagenome library containing all kinds of environmental genomic DNA. The activity-based screening was determined the protease activity on 0.5% skim milk. For sequence-based screening, we designed a set of primer expanding gene sequence of fibrinolytic enzyme, performed PCR and selected clones showing the expected size of amplicons from metagenome library. Transformation of the gene encoding fibrinolytic enzyme was carried out with commercial vectors and their transformants were selected. Finally, we found 15 positive clones from metagenome library. Then each of sequences were analyzed and identified as similar or known the clones of nattokinase. We are going to perform full sequence of each clones, ligate with expression vector, transform into competent cells and then determine activity of expressed enzymes.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Allsop, A. E. 1998. New antibiotic discovery, novel screens, novel targets and impact of microbial genomics. Curr. Opin. Microbiol. 1(5), 530-534.
Astrup, T. and S. Millertz. 1952. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40, 346-351.
Ehrilich, H. J., N. U. Bang, S. P. Little, S. R. Jaskunas, B. J. Weigel, Lo E. Mattler and C. S. Harms. 1987. Biolobical properties of a kringless tissue plasminogen activator mutant. Fibrinolysis 1, 75-77.
Handelsman, J., M. R. Rondon, S. F. Brady, J. Clardy and R. M. Goodman. 1998. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 5(10), 245-249.
Hugenholtz, P. and N. R. Pace. 1996. Identifying microbial diversity in the natural environment: a molecular phylogenetic approach. Trends Biotechnol. 14, 190-197.
Astrup, T. and S. Millertz. 1952. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40, 346-351.
Ehrilich, H. J., N. U. Bang, S. P. Little, S. R. Jaskunas, B. J. Weigel, Lo E. Mattler and C. S. Harms. 1987. Biolobical properties of a kringless tissue plasminogen activator mutant. Fibrinolysis 1, 75-77.
Handelsman, J., M. R. Rondon, S. F. Brady, J. Clardy and R. M. Goodman. 1998. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 5(10), 245-249.
Hugenholtz, P. and N. R. Pace. 1996. Identifying microbial diversity in the natural environment: a molecular phylogenetic approach. Trends Biotechnol. 14, 190-197.
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UCI(KEPA) : I410-ECN-0101-2009-470-015599963