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Campylobacter, one of the emerging foodbornepathogens, is highly adaptable to the external environmentsby changing its morphology. In the present study, a questionof whether the whole-cell antibody would still be effective forits detection even though the morphology of C. jejuni waschanged was examined. When microaerophilic C. jejuni wasexposed to aerobic conditions for 48 h, its morphologicalchange was detected by confocal laser scanning microscope:Its morphology was confirmed as a spiral-bacilli form inmicroaerobic condition, however, as a coccoid form with alittle spiral-bacilli form, when exposed to aerobic conditions.Also, the expressions of the whole-cell proteins of C. jejuni,and the suppression or induction of newly synthesizedproteins in both aerobic and microaerobic conditions wereanalyzed by two dimensional gel electrophoresis. Additionally,immunoblotting assay with the whole cell antibody for theproteins expressed under the two conditions was performed.It was confirmed that the commercial whole-cell antibody ofC. jejuni raised in rabbit was reactive. When analyzed withMALDI-TOF MS, the expressed proteins were confirmed asflagellins. Therefore, even though the morphology changed inaerobic condition, these flagellins were expressed and workedas the eitope proteins, thus making it possible to utilize for thedevelopment of an immunosensor for real-time detection ofany kind of C. jejuni cell.

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