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학술저널
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한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제2호
발행연도
2014.1
수록면
217 - 224 (8page)

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Chicken are considered as the most important source of human infection by Campylobacterjejuni, which primarily arises from contaminated poultry meats. However, the genes expressedin vivo of the interaction between chicken and C. jejuni have not been screened. In this regard,in vivo-induced antigen technology (IVIAT) was applied to identify expressed genes in vivoduring interaction between chicken and C. jejuni, a prevalent foodborne pathogen worldwide. Chicken sera were obtained by inoculating C. jejuni NCTC 11168 into Leghorn chickensthrough oral and intramuscular administration. Pooled chicken sera, adsorbed against in vitrogrowncultures of C. jejuni, were used to screen the inducible expression library of genomicproteins from sequenced C. jejuni NCTC 11168. Finally, 28 unique genes expressed in vivo weresuccessfully identified after secondary and tertiary screenings with IVIAT. The genes wereimplicated in metabolism, molecular biosynthesis, genetic information processing, transport,regulation and other processes, in addition to Cj0092, with unknown function. Severalpotential virulence-associated genes were found to be expressed in vivo, including chuA, flgS,cheA, rplA, and Cj0190c. We selected four genes with different functions to compare theirexpression levels in vivo and in vitro using real-time RT-PCR. The results indicated that theseselected genes were significantly upregulated in vivo but not in vitro. In short, the expressedgenes in vivo may act as potential virulence-associated genes, the protein encoded by whichmay be meaningful vaccine candidate antigens for campylobacteriosis. IVIAT provides animportant and efficient strategy for understanding the interaction mechanisms betweenCampylobacter and hosts.

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