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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제3호
- 발행연도
- 2014.1
- 수록면
- 363 - 370 (8page)
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초록· 키워드
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Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C.
jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play animportant role in the pathogenicity of C. jejuni. We applied an immunoscreening method, invivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during humaninfection by C. jejuni. An inducible expression library of genomic proteins was constructedfrom sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled humansera obtained from clinical patients. We successfully identified 24 unique genes expressed invivo. These genes were implicated in metabolism, molecular biosynthesis, genetic informationprocessing, transport, and other processes. We selected six genes with different functions tocompare their expression levels in vivo and in vitro using real-time RT-PCR. The resultsshowed that the selected six genes were significantly upregulated in vivo but not in vitro. Inshort, these identified in vivo-induced genes may contribute to human infection of C. jejuni,some of which may be meaningful vaccine candidate antigens or diagnosis serologic markersfor campylobacteriosis. IVIAT may present a significant and efficient method forunderstanding the pathogenicity mechanism of Campylobacter and for finding targets for itsprevention and control.
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참고문헌 (22)
참고문헌 신청
Balaban M / 2009 / FlhF and its GTPase activity are required for distinct processes in flagellar gene regulation and biosynthesis in Campylobacter jejuni / J. Bacteriol 191 :
Cordwell SJ / 2008 / Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies / Proteomics 8 : 122 ~ 139
Dasti JI / 2010 / Campylobacter jejuni: a brief overview on pathogenicityassociated factors and disease-mediating mechanisms / Int. J. Med. Microbiol 300 : 205 ~ 211
Gaasbeek EJ / 2009 / Functional characterization of excision repair and RecA-dependent recombinational DNA repair in Campylobacter jejuni / J. Bacteriol 191 : 3785 ~ 3793
Gu H / 2009 / Use of in vivo-induced antigen technology (IVIAT) for the identification of Streptococcus suis serotype 2 in vivo-induced bacterial protein antigens / BMC Micro
Cordwell SJ / 2008 / Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies / Proteomics 8 : 122 ~ 139
Dasti JI / 2010 / Campylobacter jejuni: a brief overview on pathogenicityassociated factors and disease-mediating mechanisms / Int. J. Med. Microbiol 300 : 205 ~ 211
Gaasbeek EJ / 2009 / Functional characterization of excision repair and RecA-dependent recombinational DNA repair in Campylobacter jejuni / J. Bacteriol 191 : 3785 ~ 3793
Gu H / 2009 / Use of in vivo-induced antigen technology (IVIAT) for the identification of Streptococcus suis serotype 2 in vivo-induced bacterial protein antigens / BMC Micro
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