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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제6호
- 발행연도
- 2005.1
- 수록면
- 1,267 - 1,272 (6page)
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초록· 키워드
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The effects of coexpression of GroEL/ES andDnaK/DnaJ/GrpE chaperones on the productivity of the solubleform of human granulocyte colony stimulating factor (hGCSF)in E. coli were examined. Recombinant hG-CSF proteinwas coexpressed with DnaK/DnaJ/GrpE or GroEL/ES chaperonesunder the control of the araB or Pzt-1 promoter, respectively.The optimal concentration of L-arabinose for the expressionof DnaK/DnaJ/GrpE was found to be 1 mg/ml. When Larabinosewas added at OD600=0.2 (early-exponential phase),soluble hG-CSF production was greatly increased. In addition,it was observed that the DnaK/DnaJ/GrpE and GroEL/ESchaperones had no synergistic effects on preventing aggregationof hG-CSF protein. Consequently, by coexpression of theDnaK/DnaJ/GrpE chaperone, the signal intensity of the hGCSFprotein band in the soluble fraction of cell lysate wasincreased from 3.5% to 13.9%, and Western blot analysis alsorevealed about a 4-5-fold increase of production of solublehG-CSF over the non-induction case of DnaK/DnaJ/GrpE.
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참고문헌 (27)
참고문헌 신청
/ 1992 / Cooperation ofGroEL/GroES and DnaK/DnaJ heat shock proteins inpreventing protein misfolding in Escherichia coli : 10341 ~ 89 10344
/ 1992 / Effect of overproductionof heat shock chaperones GroESL and DnaK on humanprocollagenase production in Escherichia coli 267 : 2849 ~ 2852
/ 1992 / Folding and oxidation ofrecombinant human granulocyte colony stimulating factorproduced in Escherichia coli : 8770 ~ 8777
/ 1994 / The ATP hydrolysis-dependentreaction cycle of the Escherichia coli Hsp70 system-DnaK 91 : 10345 ~ 91 10349
/ 1995 / DnaK/DnaJ supplementation improvesthe periplasmic production of human granulocyte colonystimulating factor in Escherichia coli 210 : 524 ~ 529
/ 1992 / Effect of overproductionof heat shock chaperones GroESL and DnaK on humanprocollagenase production in Escherichia coli 267 : 2849 ~ 2852
/ 1992 / Folding and oxidation ofrecombinant human granulocyte colony stimulating factorproduced in Escherichia coli : 8770 ~ 8777
/ 1994 / The ATP hydrolysis-dependentreaction cycle of the Escherichia coli Hsp70 system-DnaK 91 : 10345 ~ 91 10349
/ 1995 / DnaK/DnaJ supplementation improvesthe periplasmic production of human granulocyte colonystimulating factor in Escherichia coli 210 : 524 ~ 529
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