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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제1호
- 발행연도
- 2004.1
- 수록면
- 162 - 166 (5page)
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초록· 키워드
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Phage P22 tailspike is a thermostable homotrimericprotein, and temperature-sensitive folding (tsf) and globalsuppressor mutations affect its folding yields at elevatedtemperatures. We earlier suggested that the folding of thetailspike protein in Escherichia coli requires an unidentifiedmolecular chaperone. Accordingly, in the present study, theinteractions of purified DnaK, DnaJ, and GrpE heat-shockproteins with the tailspike protein were investigated duringthe translation and folding of the protein. The cotranslationaladdition of DnaJ to the tailspike protein resulted in the arrestof folding, when Dnak and GrpE were missing. However, thepresence of DnaK, DnaJ, and GrpE had no effect on thefolding yield of the tailspike protein, thus, providing evidencefor the binding of the nascent tailspike protein with DnaJprotein, a member of DnaK chaperoning cycle.
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참고문헌 (25)
참고문헌 신청
Beibinger, M / 1995 / Mutations that stabilize folding intermediates of phage P22 tailspike protein: folding in vivo and in vitro, stability, and structural context / J. Mol.
Brushier, R / 1993 / nteractions of phage P22 tailspike protein with GroE molecular chaperones during refolding in vitro / J. Biol. Chem. 268 : 2767 ~ 2772
Bukau, B / 1998 / The Hsp70 and Hsp60 chaperone machines / Cell 92 : 351 ~ 366
Danner, M / 1993 / Mechanism of phage P22 tailspike protein folding mutations / Protein Science 2 : 1869 ~ 1881
Fane, B / 1991 / Identification of global suppressors for temperature-sensitive Fig. 3. Restoration of post-translational folding by addition of DnaK and GrpE., folding mutat
Brushier, R / 1993 / nteractions of phage P22 tailspike protein with GroE molecular chaperones during refolding in vitro / J. Biol. Chem. 268 : 2767 ~ 2772
Bukau, B / 1998 / The Hsp70 and Hsp60 chaperone machines / Cell 92 : 351 ~ 366
Danner, M / 1993 / Mechanism of phage P22 tailspike protein folding mutations / Protein Science 2 : 1869 ~ 1881
Fane, B / 1991 / Identification of global suppressors for temperature-sensitive Fig. 3. Restoration of post-translational folding by addition of DnaK and GrpE., folding mutat
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