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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 190 - 193 (4page)
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CMP-Neu5Ac synthetase is a key enzyme for the synthesis of CMP-Neu5Ac, which is an essential precursor of sialylated glycoconjugates. For the soluble expression of the CMP-Neu5Ac synthetase gene (neuA) from Escherichia coli K1, various heat shock proteins were coexpressed in E. coli BL21 (DE3) Star. In order to do this, a pG-KJE8 plasmid encoding genes for GroEL-ES and DnaK-DnaJ-GrpE was co-transformed with neuA and was expressed at 20°C by addition of 0.01 mM IPTG and 0.005 mg/mL L-arabinose. The coexpression of various heat shock proteins resulted in remarkably improved production of soluble CMP-Neu5Ac synthetase in E. coli.
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참고문헌 (11)
참고문헌 신청
Boehm G / 2007 / Oligosaccharides from milk / J. Nutr 137 : 847 ~ 849
Gilbert M / 1997 / Characterization of a recombinant Neisseria meningitidis alpha-2,3-sialyltransferase and its acceptor specificity / Eur. J. Biochem 249 : 187 ~ 194
Lee WH / 2012 / Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-L-fucose production in recombinant Escherichia coli / Appl. Microbiol. Biotechnol 93 :
Mogk A / 1999 / Identification of thermolabile Escherichia coli proteins: prevention and reversion of aggregation by DnaK and ClpB / Embo. J 18 : 6934 ~ 6949
Nakano T / 2001 / Sialic acid in human milk: composition and functions / Acta Paediatr Taiwan 42 : 11 ~ 17
Gilbert M / 1997 / Characterization of a recombinant Neisseria meningitidis alpha-2,3-sialyltransferase and its acceptor specificity / Eur. J. Biochem 249 : 187 ~ 194
Lee WH / 2012 / Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-L-fucose production in recombinant Escherichia coli / Appl. Microbiol. Biotechnol 93 :
Mogk A / 1999 / Identification of thermolabile Escherichia coli proteins: prevention and reversion of aggregation by DnaK and ClpB / Embo. J 18 : 6934 ~ 6949
Nakano T / 2001 / Sialic acid in human milk: composition and functions / Acta Paediatr Taiwan 42 : 11 ~ 17
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