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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 212 - 218 (7page)
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Fucosyltransferases (FucTs) catalyze fucosyl transfer from guanosine-diphosphate fucose (GDP-β-L-fucose) to acceptor molecules to form fucosyloligosaccharides with α-glycosidic linkages. However, when FucT genes have been expressed in Escherichia coli, most cases have resulted in the production of inclusion bodies. In this study, to overcome this drawback, molecular chaperones were co-expressed with α1,2-fucosyltransferase (FucT2) in E. coli. For this, the pACYC184 vector, having genes for chaperones such as GroEL, GroES, DnaK, DnaJ, and GrpE, were transformed into E. coli BL21 (DE3) star harboring pHFucT2, including the FucT2 gene from Helicobacter pylori 26695. The results from SDS-PAGE showed that 5 chaperones were successfully expressed and the soluble fraction of FucT2 was also increased. HPLC analysis revealed that the coexpression of chaperone proteins resulted in a 5-fold increase in the total activity of fucosyltransferase in E. coli. In conclusion, the FucT2 expression system developed in this study can be used as a useful tool for the synthesis of fucosyloligosaccharides.
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참고문헌 (23)
참고문헌 신청
Anderson K / 2000 / Diphenylamine-anilinephosphoric acid reagent, a versatile spray reagent for revealing glycoconjugates on thin-layer chromatography plates / Anal. Biochem
Appelmelk BJ / 1996 / Potential role of molecular mimicry between Helicobacter pylori lipopolysaccharide and host Lewis blood group antigens in autoimmunity / Infect Immun 64
Baneyx F / 1999 / Recombinant protein expression in Escherichia coli / Curr. Opin. Biotechnol 10 : 411 ~ 421
Becker DJ / 2003 / Fucose: biosynthesis and biological function in mammals / Glycobiology 13 : 41 ~ 53
Chen R / 2012 / Bacterial expression systems for recombinant protein production: E. coli and beyond / Biotechnol. Adv 30 : 1102 ~ 1107
Appelmelk BJ / 1996 / Potential role of molecular mimicry between Helicobacter pylori lipopolysaccharide and host Lewis blood group antigens in autoimmunity / Infect Immun 64
Baneyx F / 1999 / Recombinant protein expression in Escherichia coli / Curr. Opin. Biotechnol 10 : 411 ~ 421
Becker DJ / 2003 / Fucose: biosynthesis and biological function in mammals / Glycobiology 13 : 41 ~ 53
Chen R / 2012 / Bacterial expression systems for recombinant protein production: E. coli and beyond / Biotechnol. Adv 30 : 1102 ~ 1107
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