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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제5호
- 발행연도
- 2007.1
- 수록면
- 792 - 799 (8page)
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초록· 키워드
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A gene encoding a putative glycogen-debranchingenzyme in Sulfolobus shibatae (abreviated as SSGDE) wascloned and expressed in Escherichia coli. The recombinantenzyme was purified to homogeneity by heat treatment andNi-NTA affinity chromatography. The recombinant SSGDEwas extremely thermostable, with an optimal temperature at85oC. The enzyme had an optimum pH of 5.5 and was highlysuggested that it possesses characteristics of both amylo-1,6-glucosidase and α-1,4-glucanotransferase. SSGDE clearlyhydrolyzed pullulan to maltotriose, and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) to maltose and β-cyclodextrin. Atthe same time, SSGDE transferred maltooligosyl residues tothe maltoligosaccharides employed, and maltosyl residues toG2-βfollowed in a decreasing order by glycogen, pullulan, andamylose. Therefore, the present results suggest that the glycogen-debranching enzyme from S. shibatae may have industrialapplication for the efficient debranching and modification ofstarch to dextrins at a high temperature.
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참고문헌 (26)
참고문헌 신청
/ 1971 / Fate of transforming DNA following uptake by competent Bacillus subtilis : 209 ~ 221
Altschul, S. F / 1997 / Gapped BLAST and PSI-BLAST: A new generation of protein database search programs 25 : 3389 ~ 3402
Bae, S. S / 2006 / Thermococcus onnurineus sp. nov., a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent area at the PACMANUS field 16 : 1826 ~ 1831
Bates / 1975181-185 / Debranching enzyme from rabbit skeletal muscle Evidence for the location of two active centers on a single polypeptide chain
Bradford / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding : 248 ~ 254
Altschul, S. F / 1997 / Gapped BLAST and PSI-BLAST: A new generation of protein database search programs 25 : 3389 ~ 3402
Bae, S. S / 2006 / Thermococcus onnurineus sp. nov., a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent area at the PACMANUS field 16 : 1826 ~ 1831
Bates / 1975181-185 / Debranching enzyme from rabbit skeletal muscle Evidence for the location of two active centers on a single polypeptide chain
Bradford / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding : 248 ~ 254
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