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논문 기본 정보
- 자료유형
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- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제3호
- 발행연도
- 2019.1
- 수록면
- 357 - 366 (10page)
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We first confirmed the involvement of MalQ (4-α-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays. In vivo tests of the knock-out mutant, ΔmalQ, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation. In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-β-CD: G4-β-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ. In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ. The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP. In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ. The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP. Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation. The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.
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참고문헌 (36)
참고문헌 신청
Carlson GM / 2018 / Novel insight into brain glycogen metabolism / J. Biol. Chem 293:7078~7088
Romeo T / 1990 / Genetic regulation of glycogen biosynthesis in Escherichia coli : in vivo effects of the catabolite repression and stringent response systems in glg gene expression / Curr. Microbiol 21:131~137
Boos W / 1998 / Maltose/maltodextrin system of Escherichia coli : transport, metabolism and regulation / Microbiol. Mol. Biol Rev 62:204~229
Ball SG / 2003 / From bacterial glycogen to starch : understanding the biogenesis of the plant starch granule / Annu. Rev. Plant Biol 54:207~233
Wilson WA / 2010 / Regulation of glycogen metabolism in yeast and bacteria / FEMS Microbiol. Rev 34:952~985
Romeo T / 1990 / Genetic regulation of glycogen biosynthesis in Escherichia coli : in vivo effects of the catabolite repression and stringent response systems in glg gene expression / Curr. Microbiol 21:131~137
Boos W / 1998 / Maltose/maltodextrin system of Escherichia coli : transport, metabolism and regulation / Microbiol. Mol. Biol Rev 62:204~229
Ball SG / 2003 / From bacterial glycogen to starch : understanding the biogenesis of the plant starch granule / Annu. Rev. Plant Biol 54:207~233
Wilson WA / 2010 / Regulation of glycogen metabolism in yeast and bacteria / FEMS Microbiol. Rev 34:952~985
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