지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제7호
- 발행연도
- 2006.1
- 수록면
- 1,104 - 1,110 (7page)
이용수
초록· 키워드
오류제보하기
that binds and kills a target cel expressing a specific antigenon the surface of the cell, and its structure is chimeric, inwhich a toxin is fused to an antigen-binding domain such asscFv or Fab. Divalent antibody-toxin molecules showed highercytotoxicities against cancer cel lines than monovalentmolecules. However, the yields of the divalent moleculeswere very low. In this study, we introduced the CH2, CH3, orCH2-CH3 (=Fc) domain of antibody in the midle of the Fab-domain to increase the yield. The covalently bonded dimercould be formed by thre disulfide bridges from cysteineresidues in the hinge region. The molecule with the CH3domain showed about 3-fold higher dimerization yield thanpreviously constructed Fab-toxin molecules, while maintainingthe cytoxic activity comparable to that of scFv-toxin.However, the introduction of CH2 or Fc domain to the sameposition showed little effect on the dimerization yield. Weposible to form noncovalently associated dimer molecules.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Baluna, R / 2000 / The effect of a monoclonal antibody coupled to ricin A chain-derived peptides on endothelial cells in vitro: Insights into toxin-mediated vascular damage /
Bera, T. K / 1998 / Comparison of recombinant immunotoxins against LeY antigen expressing tumor cells: Influence of affinity, size, and stability / Bioconjug. Chem. 9 : 736 ~
Brinkmann, U / 1991 / B3(Fv)-PE38KDEL, a single-chain immunotoxin that causes complete regression of a human carcinoma in mice / Proc. Natl. Acad. Sci. USA 88 : 8616 ~ 8620
Buchner, J / 1991 / Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli / Bio/Technology 9 : 157 ~ 162
Buchner, J / 1992 / A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodie
Bera, T. K / 1998 / Comparison of recombinant immunotoxins against LeY antigen expressing tumor cells: Influence of affinity, size, and stability / Bioconjug. Chem. 9 : 736 ~
Brinkmann, U / 1991 / B3(Fv)-PE38KDEL, a single-chain immunotoxin that causes complete regression of a human carcinoma in mice / Proc. Natl. Acad. Sci. USA 88 : 8616 ~ 8620
Buchner, J / 1991 / Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli / Bio/Technology 9 : 157 ~ 162
Buchner, J / 1992 / A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodie
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