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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제1호
- 발행연도
- 2009.1
- 수록면
- 108 - 112 (5page)
이용수
초록· 키워드
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A simplified method for the purification of cholera toxin was
developed. The 569B strain of Vibrio cholerae, a recognized
hyper-producer of cholera toxin, was propagated in a
bioreactor under conditions that promote the production of
the toxin. The toxin was separated from the bacterial cells using
0.2-µm crossflow microfiltration, the clarified toxin was passed
through the membrane into the permeate, and the bacterial
cells were retained in the retentate. The 0.2-µm permeate was
then concentrated 3-fold and diafiltered against 10 mM
phosphate buffer, pH7.6, using 30-kDa crossflow ultrafiltration.
The concentrated toxin was loaded onto a cation exchange
column, the toxin was bound to the column, and most of the
impurities were passed unimpeded through the column. The
toxin was eluted with a salt gradient of phosphate buffer, pH7.0,
containing 1.0M NaCl. The peak containing the toxin was
assayed for cholera toxin and protein and the purity was
determined to be 92%. The toxin peak had a low endotoxin
level of 3.1 EU/µg of toxin. The purified toxin was used to
prepare antiserum against whole toxin, which was used in a
GM1 ganglioside-binding ELISA to determine residual levels
of toxin in an oral inactivated whole-cell cholera vaccine.
The GM1 ganglioside-binding ELISA was shown to be very
sensitive and capable of detecting as little as 1 ng/ml of
cholera toxin.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Anh, D. D. / 2007 / Safety and immunogenicity of a reformulated Vietnamese bivalent killed, whole-cell, oral cholera vaccine in adults / Vaccine 25 : 1149 ~ 1155
Clemens, J. / 1990 / Field trial of oral cholera vaccine in Bangladesh: Results from three-year follow-up / Lancet 335 : 270 ~ 273
Cuatrecasas, P. / 1973 / Vibrio cholerae choleragenoid. Mechanism of inhibition of cholera toxin action / Biochemistry 15 : 3577 ~ 3781
Dawson, R. M. / 2005 / Characterization of the binding of cholera toxin to ganglioside GM1 immobilized onto microtitre plates / J. Appl. Toxicol 25 : 30 ~ 38
Gill, D. M. / 1976 / The arrangement of subunits in cholera toxin / Biochemistry 15 : 1242 ~ 1248
Clemens, J. / 1990 / Field trial of oral cholera vaccine in Bangladesh: Results from three-year follow-up / Lancet 335 : 270 ~ 273
Cuatrecasas, P. / 1973 / Vibrio cholerae choleragenoid. Mechanism of inhibition of cholera toxin action / Biochemistry 15 : 3577 ~ 3781
Dawson, R. M. / 2005 / Characterization of the binding of cholera toxin to ganglioside GM1 immobilized onto microtitre plates / J. Appl. Toxicol 25 : 30 ~ 38
Gill, D. M. / 1976 / The arrangement of subunits in cholera toxin / Biochemistry 15 : 1242 ~ 1248
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