지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.1
- 수록면
- 421 - 430 (10page)
이용수
초록· 키워드
오류제보하기
CDK2 and CDK4 known promoter of cel cycling catalyze phosphorylation of RB protein. Enzyme specificity betwen two CDKs that work at a diferent cel cycle phase is not clearly under-stod. In order to define kinase properties of CDK2 and CDK4 in complex with cycline A or cycline D1 in relation to their respective role in cell cy-ties of both CDK4/cycline D1 and CDK2/cycline A in vitro. Asociation constant, Km for ATP in CDK4/cyclin D1 was found as 418 M, a value un-usualy high whereas CDK2/cyclin A was 23 M, a value close to most of other regulatory protein kinases. Turnover value for both CDK4/cyclin D1 and CDK2/cyclin A were estimated as 3.4 and 3.9 min-1 respectively. Kinetic efficiency estimation in-dicates far over one order magnitude less effici-cycline A (9.3 pM-1 min-1 and 170 pM-1 min-1 respec-tively). In addition, inhibition of celular CDK4 caused increase of cellular levels of ATP, even though inhibition of CDK2 did not change it notice-ably. These data sugest celular CDK4/ cyclin D1 activity is tightly associated with celular ATP concentration. Also, analysis of phosphorylated serine/threonine sites on RB catalyzed by CDK4/ cyclin D1 and CDK2/cyclin A showed significant diferences in their preference of phosphorylation to regulate various celular proteins by binding and this binding is controled by its phosphoryl-ation, these data shown here clearly indicate signi-ficant diference in their biochemical properties betwen CDK4/cyclin D1 and CDK2/cyclin A affec-ting regulation of celular RB function.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Gene expression regulation by CDK12: a versatile kinase in cancer with functions beyond CTD phosphorylation
Experimental and Molecular Medicine
2020 .01
-
Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex
Experimental and Molecular Medicine
2019 .01
-
Discovery of Cyclin-dependent Kinase Inhibitor, CR229, Using Structurebased Drug Screening
Journal of Microbiology and Biotechnology
2007 .01
-
6-Aminonicotinamide Induces $G_1$ Arrest by Elevating $p27^{kip1}$ as well as Inhibiting cdk2, Cyclin E and p-Rb in IMR32 Neuroblastoma Cell Line
Integrative biosciences
2005 .01
-
택란 메탄올 추출물에 의한 인체 폐암 세포주 A549의 G1 arrest 유발
생명과학회지
2013 .09
-
Cardiac Development and Cell Cycle
한국생물물리학회 학술발표회
1998 .01
-
The function of p27KIP1 during tumor development
Experimental and Molecular Medicine
2009 .01
-
Involvement of Cdk Inhibitor p21(WIP1/CIP1) in G2/M Arrest of Human Myeloid Leukemia U937 Cells by N-Methyl-N'-Nitro-N-Nitrosoguanidine
생명과학회지
2009 .01
-
Regulation of Cyclin D3 by Calpain Protease in Human Breast Carcinoma MDA-MB-231 Cells
생명과학회지
2006 .07
-
G1 Arrest of U937 Human Monocytic Leukemia Cells by Sodium Butyrate, an HDAC Inhibitor, Via Induction of Cdk Inhibitors and Down-regulation of pRB Phosphorylation
생명과학회지
2009 .07
-
Calpain protease에 의한 cyclin D3의 post-translation조절
생명과학회지
2015 .01
-
Increase of Cdk5 and p35 during Retinoic Acid-Induced Neuronal Differentiation of SK-N-BE(2)C cells
한국생물물리학회 학술발표회
2002 .01
-
CDK5-dependent inhibitory phosphorylation of Drp1 during neuronal maturation
Experimental and Molecular Medicine
2014 .01
-
Inhibition of pRB Phosphorylation and Induction of p21WAF1/CIP1 Occur During cAMP-induced Growth Arrest in Human Neuroblastoma Cells
생명과학회지
2003 .10
-
Induction of Cell Cycle Arrest by Cyclic AMP in Tumor Cells through p53-independent Transcriptional Regulation of Cdk inhibitor p21WAF1/CIP1
한국동물학회 학술대회
2001 .01
-
Cordycepin에 의한 LNCap 인체 전립선 암세포의 apoptosis 및 G2/M arrest 유발
생명과학회지
2014 .01
-
Expression of Cyclin D3 Transcripts in the Postmeiotic Male Germ Cells of the Mouse
Korean journal of biological sciences
1998 .01
-
Synergistic Effects of Dexamethasone and Genistein on the Expression of Cdk inhibitor p21WAF1/CIP1 in Human Hepatocellular and Colorectal Carcinoma Cells
한국생명과학회 학술발표회
2001 .01
-
TSPAN12 Precedes Tumor Proliferation by Cell Cycle Control in Ovarian Cancer
Molecules and Cells
2019 .01
-
Resveratrol에 의한 A549 인체 폐암세포의 증식억제 및 apoptosis 유발에 관한 연구
생명과학회지
2004 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0