지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제16권 제3호
- 발행연도
- 2015.1
- 수록면
- 104 - 108 (5page)
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초록· 키워드
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Mycoplasma hyorhinis (M. hyorhinis) is considered an etiological agent of arthritis in suckling pigs. Recently, some M. hyorhinis strains were shown to produce pneumonia that is indistinguishable from the mycoplasmosis caused by M. hyopneumoniae. In this study, we developed a sensitive and specific PCR assay to detect M. hyorhinis and applied the developed PCR assay for detection of Mycoplasma infection in clinical piglets infected with M. hyorhinis. We developed a new PCR assay using a M. hyorhinis-specific primer pair, Mrhin-F and Mrhin-R, designed from the Mycoplasma 16S-23S rRNA internal transcribed spacer (ITS) region. The primers and probe for the assay were designed from regions in the Mycoplasma 16S-23S rRNA ITS unique to M. hyorhinis. The developed PCR assay was very specific and sensitive for the detection of M. hyorhinis. The assay could detect the equivalent of 1 pg of target template DNA, which indicates that the assay was very sensitive. In addition, M. hyorhinis PCR assay detected only M. hyorhinis and not any other Mycoplasma or bacterial spp. of other genera. The new developed PCR assay effectively detected M. hyorhinis infection in pigs. We suggest that this PCR assay using a M. hyorhinis-specific primer pair, Mrhin-F and Mrhin-R, could be useful and effective for monitoring M. hyorhinis infection in pigs.
목차
등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Amass SF / 1994 / Interaction of Mycoplasma hyopneumoniae and Pasteurella multocida infections in swine / JAVMA 204 : 102 ~ 107
Brown D / 2007 / Revised minimal standards for description of new species of the class Mollicutes(division Tenericutes) / Int J Syst Evol Microbiol 57 : 2703 ~ 2719
Calsamiglia, M / 2000 / Correlation between the presence of enzootic pneumoniae lesions and detection of Mycoplasma hyopneumoniae in bronchial swabs by PCR / Vet Microbiol 7
Dudler R / 1988 / A mycoplasma high-affinity transport system and the in vitro invasiveness of mouse sarcoma cells / EMBO J 37 : 119 ~ 128
Friis NF / 1971 / Mycoplasma hyorhinis as a causative agent in arthritis of pigs / Acta Vet Scand 12 : 116 ~ 119
Brown D / 2007 / Revised minimal standards for description of new species of the class Mollicutes(division Tenericutes) / Int J Syst Evol Microbiol 57 : 2703 ~ 2719
Calsamiglia, M / 2000 / Correlation between the presence of enzootic pneumoniae lesions and detection of Mycoplasma hyopneumoniae in bronchial swabs by PCR / Vet Microbiol 7
Dudler R / 1988 / A mycoplasma high-affinity transport system and the in vitro invasiveness of mouse sarcoma cells / EMBO J 37 : 119 ~ 128
Friis NF / 1971 / Mycoplasma hyorhinis as a causative agent in arthritis of pigs / Acta Vet Scand 12 : 116 ~ 119
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