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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 101 - 106 (6page)
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초록· 키워드
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Murine mycoplasmosis, caused by Mycoplasma (M.) pulmonis, is a prominent disease in rodent animals. The aim of this study was to develop a sensitive and specific PCR assay to detect M. pulmonis in ani-mals and to assess the suitability of this assay for the detection of mycoplasmal infection in rats ex-perimentally infected with M. pulmonis. A new PCR assay using the M. pulmonis-specific primer pairs MPul-F and MPul-R was developed. The primers and probe for the assay were designed from regions in the 16S rRNA gene that are unique to M. pulmonis. The novel PCR assay was very specific and sensitive for M. pulmonis, detecting the equivalent of 5 pg of target template DNA. It detected only M. pulmonis and no other Mycoplasma species or other bacterial species. The newly developed PCR assay also effectively detected M. pulmonis infection in rats. These results suggest that this PCR assay using M. pulmonis-specific primer pairs of MPul-F and MPul-R will be useful and effective for monitor-ing M. pulmonis infection in animals.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Nicholas RA / 1996 / A comparison of serological tests and gross lung pathology for detecting contagious bovine pleuropneumonia in two groups of Italian cattle / Vet Rec 139:89~93
Nicklas W / 1993 / Contamination of transplantable tumors, cell lines, and monoclonal antibodies with rodent viruses / Lab Anim Sci 43:296~300
Armstrong CH / 1987 / Crossreactions between Mycoplasma hyopneumoniae and Mycoplasma flocculare : practical implications for the serodiagnosis of mycoplasmal pneumonia of swi
Baker DG / 1998 / Natural pathogens of laboratory mice, rats, and rabbits and their effects on research / Clin Microbiol Rev 11 : 231 ~ 266
Ball HJ / 1998 / Diagnostic application of monoclonal antibody(MAb)-based sandwich ELISAs / Methods Mol Biol 104 : 127 ~ 132
Nicklas W / 1993 / Contamination of transplantable tumors, cell lines, and monoclonal antibodies with rodent viruses / Lab Anim Sci 43:296~300
Armstrong CH / 1987 / Crossreactions between Mycoplasma hyopneumoniae and Mycoplasma flocculare : practical implications for the serodiagnosis of mycoplasmal pneumonia of swi
Baker DG / 1998 / Natural pathogens of laboratory mice, rats, and rabbits and their effects on research / Clin Microbiol Rev 11 : 231 ~ 266
Ball HJ / 1998 / Diagnostic application of monoclonal antibody(MAb)-based sandwich ELISAs / Methods Mol Biol 104 : 127 ~ 132
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