지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 995 - 1,003 (9page)
이용수
초록· 키워드
오류제보하기
Purpose: The cyclin-dependent kinase 1 (Cdk1) and cyclin B complex performs important roles in the transition from the G2 to Mphase in the cell cycle through removal of inhibitory phosphates on Cdk1, and Cdc25B, which is a dual-specific phosphatase, mediatesthese dephosphorylation events. However, measuring Cdc25B activity by existing methods is hampered by inadequatenonspecific substrates and the need to use a radiolabeled isotope. The present study aimed to develop an improved method withwhich to properly measure Cdc25B activity using a novel nonradioisotopic assay and Cdc25B overexpression cell lines. Materials and Methods: A nonradioisotopic Cdk1 kinase assay, based on Western blotting for retinoblastoma protein and histoneH1, was used to analyze Cdc25B activity. Also, stable Cdc25B2 and Cdc25B3 overexpression HeLa cell lines were constructedusing the tetracycline-regulated expression system and were applied as a tool for screening for inhibitors of Cdc25B. Results: The present study developed and optimized a nonradioisotopic assay method to properly measure Cdc25B activity. Furthermore,we constructed stable Cdc25B2 and Cdc25B3 overexpression HeLa cell lines for the establishment of a strong assay systemwith which to evaluate the specificity of Cdc25B inhibitors under conditions similar to the intracellular environment. Thesemethods were confirmed as useful tools for measuring Cdc25B activity. Conclusion: The nonradioisotopic Cdk1 kinase assay and Cdc25B overexpression cell lines developed in this study can be convenientlyused as tools for screening inhibitors of Cdc25B phosphatase as anticancer drugs.
목차
등록된 정보가 없습니다.
참고문헌 (33)
참고문헌 신청
Boutros R / 2007 / CDC25 phosphatases in cancer cells: key players? Good targets? / Nat Rev Cancer 7:495~507
Yu L / 1998 / UCN-01 abrogates G2 arrest through a Cdc2-dependent pathway that is associated with inactivation of the Wee1Hu kinase and activation of the Cdc25C phosphatase / J Biol Chem 273:33455~33464
Sur S / 2016 / Phosphatases and kinases regulating CDC25 activity in the cell cycle: clinical implications of CDC25 overexpression and potential treatment strategies / Mol Cell Biochem 416:33~46
Gabrielli BG / 1996 / Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells / J Cell Sci 109:1081~1093
Lammer C / 1998 / The cdc25B phosphatase is essential for the G2/M phase transition in human cells / J Cell Sci 111:2445~2453
Yu L / 1998 / UCN-01 abrogates G2 arrest through a Cdc2-dependent pathway that is associated with inactivation of the Wee1Hu kinase and activation of the Cdc25C phosphatase / J Biol Chem 273:33455~33464
Sur S / 2016 / Phosphatases and kinases regulating CDC25 activity in the cell cycle: clinical implications of CDC25 overexpression and potential treatment strategies / Mol Cell Biochem 416:33~46
Gabrielli BG / 1996 / Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells / J Cell Sci 109:1081~1093
Lammer C / 1998 / The cdc25B phosphatase is essential for the G2/M phase transition in human cells / J Cell Sci 111:2445~2453
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Identification of stemness and differentially expressed genes in human cementum-derived cells
Journal of Periodontal & Implant Science
2021 .10
-
Upregulation of lnc‑FOXD2‑AS1, CDC45, and CDK1 in patients with primary non‑M3 AML is associated with a worse prognosis
Blood Research
2024 .03
-
MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Helicobacter pylori inhibited cell proliferation in human periodontal ligament fibroblasts through the Cdc25C/CDK1/cyclinB1 signaling cascade
Journal of Periodontal & Implant Science
2019 .01
-
제주도 성인의 신체활동 수준
보건교육건강증진학회지
2019 .10
-
Cell Division Cycle 2 Protects Neonatal Rats Against Hyperoxia-Induced Bronchopulmonary Dysplasia
Yonsei Medical Journal
2020 .01
-
TCF4-Targeting miR-124 is Differentially Expressed amongst Dendritic Cell Subsets
Immune Network
2016 .02
-
Health Indicators Related to Disease, Death, and Reproduction
예방의학회지
2019 .01
-
p21-activated kinase 1 is allostericallyInhibited by naphthoquinone (NQ) derivatives
Journal of Biomedical and Translational Research
2017 .06
-
Comparison of the Effectiveness and Clinical Outcome of Everolimus Followed by CDK4/6 Inhibitors with the Opposite Treatment Sequence in Hormone Receptor-Positive, HER2-Negative Metastatic Breast Cancer
Cancer Research and Treatment
2022 .04
-
A Simple and Rapid Method Based on Liquid Chromatography–Tandem Mass Spectrometry for the Measurement of α-L-Iduronidase Activity in Dried Blood Spots: An Application to Mucopolysaccharidosis I (Hurler) Screening
Annals of Laboratory Medicine
2015 .01
-
Pre-immigration Screening for Tuberculosis in South Korea: A Comparison of Smear- and Culture-Based Protocols
Tuberculosis and Respiratory Diseases
2019 .01
-
Time to Chemotherapy for Patients With Estrogen Receptor-Positive Breast Cancer and Cyclin-Dependent Kinase 4 and 6 Inhibitor Use
Journal of Breast Cancer
2022 .08
-
황금 에탄올 추출물에 의한 인간 신장암 세포주 Caki-1의 G2/M arrest 유발
대한한의학방제학회지
2015 .01
-
Inhibition of ATR Increases the Sensitivity to WEE1 Inhibitor in Biliary Tract Cancer
Cancer Research and Treatment
2020 .01
-
Intraoperative infection prevention measures for surgical procedures: Lessons from the MERS outbreak
대한임상종양학회 학술대회지
2021 .06
-
Enrichment of Wee1/CDC2 and NF-κB Signaling Pathway Constituents Mutually Contributes to CDDP Resistance in Human Osteosarcoma
Cancer Research and Treatment
2022 .01
-
Overexpression of Cell Cycle Proteins of Peripheral Lymphocytes in Patients with Alzheimer’s Disease
PSYCHIATRY INVESTIGATION
2016 .01
-
The Assessment and Outcomes of Crossmatching in Lung Transplantation in Korean Patients
Journal of Korean Medical Science
2022 .06
-
Cellular Effects of Troglitazone on YD15 Tongue Carcinoma Cells
International Journal of Oral Biology
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0