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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제51권 제5호
- 발행연도
- 2021.10
- 수록면
- 329 - 341 (13page)
- DOI
- 10.5051/jpis.2102600130
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Purpose: Periodontal treatment aims at complete regeneration of the periodontium, and developing strategies for periodontal regeneration requires a deep understanding of the tissues composing the periodontium. In the present study, the stemness characteristics and gene expression profiles of cementum-derived cells (CDCs) were investigated and compared with previously established human stem cells. Candidate marker proteins for CDCs were also explored.
Methods: Periodontal ligament stem cells (PDLSCs), pulp stem cells (PULPSCs), and CDCs were isolated and cultured from extracted human mandibular third molars. Human bone marrow stem cells (BMSCs) were used as a positive control. To identify the stemness of CDCs, cell differentiation (osteogenic, adipogenic, and chondrogenic) and surface antigens were evaluated through flow cytometry. The expression of cementum protein 1 (CEMP1) and cementum attachment protein (CAP) was investigated to explore marker proteins for CDCs through reverse-transcription polymerase chain reaction. To compare the gene expression profiles of the 4 cell types, mRNA and miRNA microarray analysis of 10 samples of BMSCs (n=1), PDLSCs (n=3), PULPSCs (n=3), and CDCs (n=3) were performed.
Results: The expression of mesenchymal stem cell markers with a concomitant absence of hematopoietic markers was observed in PDLSCs, PULPSCs, CDCs and BMSCs. All 4 cell populations also showed differentiation into osteogenic, adipogenic, and chondrogenic lineages. CEMP1 was strongly expressed in CDCs, while it was weakly detected in the other 3 cell populations. Meanwhile, CAP was not found in any of the 4 cell populations. The mRNA and miRNA microarray analysis showed that 14 mRNA genes and 4 miRNA genes were differentially expressed in CDCs vs. PDLSCs and PULPSCs.
Conclusions: Within the limitations of the study, CDCs seem to have stemness and preferentially express CEMP1. Moreover, there were several up- or down-regulated genes in CDCs vs. PDLSCs, PULPSCs, and BMSCs and these genes could be candidate marker proteins of CDCs.
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참고문헌 (39)
참고문헌 신청
Gottlow J / 1986 / New attachment formation in the human periodontium by guided tissue regeneration. Case reports / J Clin Periodontol 13:604~616
Nyman S / 1982 / New attachment following surgical treatment of human periodontal disease / J Clin Periodontol 9:290~296
Tonetti MS / 1998 / Generalizability of the added benefits of guided tissue regeneration in the treatment of deep intrabony defects. Evaluation in a multicenter randomized controlled clinical trial / J Periodontol 69:1183~1192
Tonetti MS / 1996 / Factors affecting the healing response of intrabony defects following guided tissue regeneration and access flap surgery / J Clin Periodontol 23:548~556
Park JY / 2011 / Efficacy of periodontal stem cell transplantation in the treatment of advanced periodontitis / Cell Transplant 20:271~285
Nyman S / 1982 / New attachment following surgical treatment of human periodontal disease / J Clin Periodontol 9:290~296
Tonetti MS / 1998 / Generalizability of the added benefits of guided tissue regeneration in the treatment of deep intrabony defects. Evaluation in a multicenter randomized controlled clinical trial / J Periodontol 69:1183~1192
Tonetti MS / 1996 / Factors affecting the healing response of intrabony defects following guided tissue regeneration and access flap surgery / J Clin Periodontol 23:548~556
Park JY / 2011 / Efficacy of periodontal stem cell transplantation in the treatment of advanced periodontitis / Cell Transplant 20:271~285
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