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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 199 - 208 (10page)
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Objectives : In the present study, we investigated the effects of ethanol extract of Scutellaria baicalensis (EESB) on the progression of cell cycle in human renal cell carcinoma Caki-1 cells. Methods : The effects of EESB on cell growth and apoptosis induction were evaluated by trypan blue dye exclusion assay and flow cytometry, respectively. The mRNA and protein levels were determined by Western blot analysis and reverse transcription-polymerase chain reaction, respectively. Results : It was found that EESB treatment on Caki-1 cells resulted in a dose-dependent inhibition of cell growth and induced apoptotic cell death as detected by Annexin V-FITC staining. The flow cytometric analysis indicated that EESB resulted in G2/M arrest in cell cycle progression which was associated with the down-regulation of cyclin A expression. Our results also revealed that treatment with EESB increased the mRNA and proteins expression of tumor suppressor p53 and cyclin-dependent kinase (Cdk) inhibitor p21(WAF1/CIP1), without any noticeable changes in cyclin B1, Cdk2 and Cdc2. In addition, the incubation of cells with EESB resulted in a significant increase in the binding of p21 and Cdk2 and Cdc2. These findings suggest that EESB-induced G2/M arrest and apoptosis in Caki-1 cells is mediated through the p53-mediated upregulation of Cdk inhibitor p21. Conclusions : Taken together, these findings suggest that EESB may be a potential chemotherapeutic agent and further studies will be needed to identify the biological active compounds that confer the anti-cancer activity of S. baicalensis.
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참고문헌 (33)
참고문헌 신청
Coqueret O. / 2003 / New roles for p21 and p27cell-cycle inhibitors: a function for each cell compartment? / Trends Cell Biol 13:65~70
Bohn OL / 2009 / Tumor-to-tumor metastasis: Renal cell carcinoma metastatic to papillary carcinoma of thyroid-report of a case and review of the literature / Head Neck Pathol 3:327~330
Motzer RJ / 1996 / Renal-cell carcinoma / N Engl J Med 335:865~875
Joniau S / 2006 / The indications for partial nephrectomy in the treatment of renal cell carcinoma / Nat Clin Pract Urol 3:198~205
Huang Y / 2005 / Biological properties of baicalein in cardiovascular system / Curr Drug Targets Cardiovasc Haematol Disord 5:177~184
Bohn OL / 2009 / Tumor-to-tumor metastasis: Renal cell carcinoma metastatic to papillary carcinoma of thyroid-report of a case and review of the literature / Head Neck Pathol 3:327~330
Motzer RJ / 1996 / Renal-cell carcinoma / N Engl J Med 335:865~875
Joniau S / 2006 / The indications for partial nephrectomy in the treatment of renal cell carcinoma / Nat Clin Pract Urol 3:198~205
Huang Y / 2005 / Biological properties of baicalein in cardiovascular system / Curr Drug Targets Cardiovasc Haematol Disord 5:177~184
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