지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 416 - 424 (9page)
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초록· 키워드
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Purpose: Parkinson’s disease (PD) is a common age-dependent neurodegenerative disease. MiR-212 has been demonstrated toexert protective effects in several neurological disorders. The present study aimed to investigate the role and underlying molecularmechanism of miR-212 in PD. Materials and Methods: 1-methyl-4-phenylpyridinium (MPP+)-induced SH-SY5Y cells were applied as a PD model in vitro. RTqPCRwas used to measure the expression of miR-212 and Kruppel-like factor 4 (KLF4) mRNA. Western blot analysis was performedto detect the protein levels of KLF4, Notch1 and Jagged1. Cell viability and apoptosis were determined by the Cell CountingKit-8 and flow cytometry, respectively. Quantitative analysis of caspase-3 activity, lactate dehydrogenase (LDH), reactiveoxygen species (ROS), superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α), and interleukin-1 beta (IL-1β) was conductedwith corresponding ELISA kits. Dual-luciferase reporter assay was employed to evaluate the relationship between miR-212 and KLF4. Results: MiR-212 was downregulated in MPP+-induced SH-SY5Y cells. Also, miR-212 alleviated MPP+-induced SH-SY5Y cell damage,embodied by increased cell viability, decreased caspase-3 activity, LDH release, ROS production, TNF-α, and IL-1β expression,as well as elevated SOD levels. KLF4 was a direct target of miR-212, and miR-212 repressed KLF4 expression in a post-transcriptionalmanner. Moreover, miR-212-mediated protection effects were abated following KLF4 expression restoration in MPP+-induced SH-SY5Y cells, represented as lowered cell viability and enhanced apoptotic rate. Furthermore, Notch signaling wasinvolved in the regulation of miR-212/KLF4 axis in MPP+-induced SH-SY5Y cells. Conclusion: miR-212 might attenuate MPP+-induced neuronal damage by regulating KLF4/Notch signaling pathway in SH-SY5Ycells, a promising target for PD therapy.
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참고문헌 (29)
참고문헌 신청
Kalia LV / 2015 / Parkinson's disease / Lancet 386:896~912
Abeliovich A / 2016 / Defects in trafficking bridge Parkinson's disease pathology and genetics / Nature 539:207~216
Andican G / 2012 / Plasma oxidative and inflammatory markers in patients with idiopathic Parkinson's disease / Acta Neurol Belg 112:155~159
Kalia LV / 2015 / Disease-modifying strategies for Parkinson's disease / Mov Disord 30:1442~1450
Hammond SM / 2015 / An overview of microRNAs / Adv Drug Deliv Rev 87:3~14
Abeliovich A / 2016 / Defects in trafficking bridge Parkinson's disease pathology and genetics / Nature 539:207~216
Andican G / 2012 / Plasma oxidative and inflammatory markers in patients with idiopathic Parkinson's disease / Acta Neurol Belg 112:155~159
Kalia LV / 2015 / Disease-modifying strategies for Parkinson's disease / Mov Disord 30:1442~1450
Hammond SM / 2015 / An overview of microRNAs / Adv Drug Deliv Rev 87:3~14
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