개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 790 - 794 (5page)
이용수
초록· 키워드
오류제보하기
Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediatesafe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expressionof a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simplelarge-scale preparation method that can generate 3×1013 viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Herz J / 1993 / Adenovirus-mediated transfer of low density lipoprotein receptor gene acutely accelerates cholesterol clearance in normal mice / Proc Natl Acad Sci U S A 90:2812~2816
Becker TC / 1994 / Use of recombinant adenovirus for metabolic engineering of mammalian cells / Methods Cell Biol 43(Pt A):161~189
Konz JO / 2005 / Development of a purification process for adenovirus: controlling virus aggregation to improve the clearance of host cell DNA / Biotechnol Prog 21:466~472
Yang Y / 1994 / Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy / Proc Natl Acad Sci U S A 91:4407~4411
Kozarsky KF / 1995 / Gene therapy of hypercholesterolemic disorders / Trends Cardiovasc Med 5:205~209
Becker TC / 1994 / Use of recombinant adenovirus for metabolic engineering of mammalian cells / Methods Cell Biol 43(Pt A):161~189
Konz JO / 2005 / Development of a purification process for adenovirus: controlling virus aggregation to improve the clearance of host cell DNA / Biotechnol Prog 21:466~472
Yang Y / 1994 / Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy / Proc Natl Acad Sci U S A 91:4407~4411
Kozarsky KF / 1995 / Gene therapy of hypercholesterolemic disorders / Trends Cardiovasc Med 5:205~209
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Management of antineutrophil cytoplasmic antibody-associated vasculitis: a review of recent guidelines
대한류마티스학회지
2023 .04
-
Comparison in vivo knock-in strategy using dual adeno associated virus
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Adeno-Associated Virus 2-Mediated Hepatocellular Carcinoma is Very Rare in Korean Patients
Annals of Laboratory Medicine
2016 .01
-
Acute coronary syndrome in antineutrophil cytoplasmic antibody-associated vasculitis: a Korean single-centre cohort study
대한류마티스학회지
2023 .04
-
AAV delivery in in vivo HCC xenograft is different from HCC cell lines
한국실험동물학회 학술발표대회 논문집
2018 .01
-
A Novel adeno-associated virus (AAV) vector system for CRISPR/Cpf1-mediated multiplex gene editing
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Serum Clusterin Level Could Reflect the Current Activity of Antineutrophil Cytoplasmic Antibody-Associated Vasculitis
Yonsei Medical Journal
2021 .11
-
Serological Biomarkers and Indices for the Current Activity and Prognosis of ANCA-Associated Vasculitis: Experience in a Single Centre in Korea
Yonsei Medical Journal
2021 .01
-
In vivo multiplex gene targeting with Streptococcus pyogens and Campylobacter jejuni Cas9 for pancreatic cancer modeling in wild-type animal
Journal of Veterinary Science
2020 .03
-
Comparison of the 2022 ACR/EULAR Classification Criteria for Antineutrophil Cytoplasmic Antibody-Associated Vasculitis with Previous Criteria
Yonsei Medical Journal
2023 .01
-
Comparison of Radiological and Histological Findings of Lung Parenchyma in Patients with Antineutrophil Cytoplasmic Antibody-Associated Vasculitis
Yonsei Medical Journal
2019 .01
-
A mouse model for SARS-CoV-2 infection using adeno-associated virus encoding hACE2
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Classification of Antineutrophil Cytoplasmic Antibody-associated Vasculitis
대한류마티스학회지
2019 .01
-
Antineutrophil Cytoplasmic Antibody-Associated Vasculitis in Korea: A Narrative Review
Yonsei Medical Journal
2019 .01
-
AITC induces MRP1 expression by protecting against CS/CSE-mediated DJ-1 protein degradation via activation of the DJ-1/Nrf2 axis
The Korean Journal of Physiology & Pharmacology
2020 .01
-
Implication of Serious Infections in Patients With Antineutrophil Cytoplasmic Antibody-Associated Vasculitis for the First Cycle of Rituximab: A Pilot Study in a Single Korean Center
대한류마티스학회지
2023 .01
-
Efficient generation of conditional mutant mice by CRISPR RNP electroporation and AAV donor infection
한국실험동물학회 학술발표대회 논문집
2021 .07
-
The impact of prophylactic ureteral stenting during kidney transplantation on postoperative surgical outcomes
Clinical Transplantation and Research
2021 .01
-
Controlling Nutritional Status Score is Associated with All-Cause Mortality in Patients with Antineutrophil Cytoplasmic Antibody-Associated Vasculitis
Yonsei Medical Journal
2019 .01
-
잘 치유되지 않는 중이염으로 나타난 국한형 Myeloperoxidase-Antineutrophil Cytoplasmic Antibody 관련 혈관염 2예
대한이비인후-두경부외과학회지
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0