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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 203 - 203 (1page)
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Objective: CRISPR/Cpf1 is versatile genome editing tool for studying the functions of genetic elements. Electroporation (EP)-based CRISPR technologies offer significant advantages over microinjection since it can be used for a large-scale manipulation in a short period of time and delivers a variety of molecules to zygotes. Adeno-associated virus (AAV) has been recently used as a trans-zona pellucida delivery vector of DNA donor for EP-based CRISPR system. In this study, we applied CRISPR/AsCpf1 ribonucleoprotein (RNP) EP with AAV to generate knock-in mice that had an Elk-3 conditional allele. Materials and Methods: RNP was electroporated into zygotes that were infected with AAV containing a conditional allele of Elk-3 gene which serves as a long DNA donor. Electroporated zygotes were cultured in vitro to the blastocyst stage for evaluating recombination efficiency of homology-directed repair or transferred at the two-cell stage to pseudopregnant mice to obtain live pups. Integration of loxP site(s) was determi ... 전체 초록 보기
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