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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 114 - 117 (4page)
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초록· 키워드
오류제보하기
In vivo labeling of bone with fluorochromes is a widely used method for assessment of bone formation and remodeling processes. In particular, calcein is used as a marker for identification of bone growth, which is indicated by a green color. Calcein green is a calcium chelator that adheres to regions of mineralizing bone thereby allowing localization of new bone. Bone formation and remodeling in vivo can be assessed by calcium-binding calcein labeling. In this study, changes in the femoral bone of a normal mouse model at both 4 and 8 weeks were evaluated using calcein labeling. Intense deposition of calcium in the bone was observed after application for 8 weeks. A mouse model is suitable for application in in vivo experiments using genetically modified mice, such as knock-out mice, however data regarding femoral cross sectional bone in young mice are limited. The current study confirmed calcein as a useful marker for identification of bone growth, which was indicated by a green color on photomicrographs. This methodological process may provide basic information for interpreting bone formation and regeneration to pharmacologic or genetic manipulation in mice.
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등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Solheim T / 1974 / Pluricolor fluorescent labeling of mineralizing tissue / Scand J Dent Res 82:19~27
Schicker M / 2004 / The use of four-colour immunofluorescence techniques to identify mesenchymal stem cells / J Anat 204:133~139
Andre T / 1956 / Studies on the distribution of tririum-labelled dihydrostreptomycin and tetracycline in the body / Acta Radiol 142:1~89
Recker RR / 1988 / Static and tetracycline-based bone histomorphometric data from 34 normal postmenopausal females / J Bone Miner Res 3:133~144
Turner RT / 1994 / Cancellous bone turnover in growing rats : time-dependent changes in association between calcein label and osteoblasts / J Bone Miner Res 9:1419~1424
Schicker M / 2004 / The use of four-colour immunofluorescence techniques to identify mesenchymal stem cells / J Anat 204:133~139
Andre T / 1956 / Studies on the distribution of tririum-labelled dihydrostreptomycin and tetracycline in the body / Acta Radiol 142:1~89
Recker RR / 1988 / Static and tetracycline-based bone histomorphometric data from 34 normal postmenopausal females / J Bone Miner Res 3:133~144
Turner RT / 1994 / Cancellous bone turnover in growing rats : time-dependent changes in association between calcein label and osteoblasts / J Bone Miner Res 9:1419~1424
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