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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 379 - 385 (7page)
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초록· 키워드
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Purpose: Zinc (Zn) is an essential trace element for bone mineralization and osteoblast function. We examined the effects of Zn deficiency on osteoblast differentiation and mineralization in MC3T3-E1 cells. Methods: Osteoblastic MC3T3-E1 cells were cultured at concentration of 1 to 15 μM ZnCl₂ (Zn- or Zn+) for 5, 15 and 25 days up to the calcification period. Extracellular matrix mineralization was detected by staining Ca and P deposits using Alizarin Red and von Kossa stain respectively, and alkaline phosphatase (ALP) activity was detected by ALP staining and colorimetric method. Results: Extracellular matrix mineralization was decreased in Zn deficiency over 5, 15, and 25 days. Similarly, staining of ALP activity as the sign of an osteoblast differentiation, was also decreased by Zn deficiency over the same period. Interestingly, the gene expression of bone-related markers (ALP, PTHR; parathyroid hormone receptor, OPN; osteopontin, OC; osteocalcin and COLI; collagen type I), and bone-specific transcription factor Runx2 were downregulated by Zn deficiency for 5 or 15 days, however, this was restored at 25 days. Conclusion: Our data suggests that Zn deficiency inhibits osteoblast differentiation by retarding bone marker gene expression and also inhibits bone mineralization by decreasing Ca/P deposition as well as ALP activity.
목차
ABSTRACT
Introduction
Methods
Results
Discussion
Summary
References
참고문헌 (24)
참고문헌 신청
[학술지(정기간행물)] - Banerjee C / 2001 / Differential regulation of the two principal Runx2/Cbfa1 n-terminal isoforms in response to bone morphogenetic protein-2 during development of the osteoblast phenotype / Endocrinology 142 (9) : 4026 ~ 4039
[학술지(정기간행물)] - Nakashima K / 2002 / The novel zinc fingercontaining transcription factor osterix is required for osteoblast differentiation and bone formation / Cell 108 (1) : 17 ~ 29
[학술지(정기간행물)] - Prasad AS / 1995 / Zinc: an overview / Nutrition 11 (1 Suppl) : 93 ~ 99
[학술지(정기간행물)] - Leek JC / 1984 / Studies of marginal zinc deprivation in rhesus monkeys. V. Fetal and infant skeletal effects / Am J Clin Nutr 40 (6) : 1203 ~ 1212
[학술지(정기간행물)] - Yamaguchi M / 2010 / Role of nutritional zinc in the prevention of osteoporosis / Mol Cell Biochem 338 (1-2) : 241 ~ 254
[학술지(정기간행물)] - Nakashima K / 2002 / The novel zinc fingercontaining transcription factor osterix is required for osteoblast differentiation and bone formation / Cell 108 (1) : 17 ~ 29
[학술지(정기간행물)] - Prasad AS / 1995 / Zinc: an overview / Nutrition 11 (1 Suppl) : 93 ~ 99
[학술지(정기간행물)] - Leek JC / 1984 / Studies of marginal zinc deprivation in rhesus monkeys. V. Fetal and infant skeletal effects / Am J Clin Nutr 40 (6) : 1203 ~ 1212
[학술지(정기간행물)] - Yamaguchi M / 2010 / Role of nutritional zinc in the prevention of osteoporosis / Mol Cell Biochem 338 (1-2) : 241 ~ 254
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UCI(KEPA) : I410-ECN-0101-2019-594-000108874