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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.3
- 수록면
- 267 - 272 (6page)
- DOI
- 10.14348/molcells.2015.2315
이용수
초록· 키워드
오류제보하기
Nacre seashell is a natural osteoinductive biomaterial with strong effects on osteoprogenitors, osteoblasts, and osteoclasts during bone tissue formation and morphogenesis.
Although nacre has shown, in one study, to induce bridging of new bone across large non-union bone defects in 8 individual human patients, there have been no succeeding human surgical studies to confirm this outstanding potency.
But the molecular mechanisms associated with nacre osteoinduction and the influence on bone marrow-derived mesenchymal stem cells (BMSC’s), skeletal stem cells or bone marrow stromal cells remain elusive. In this study we highlight the phenotypic and biochemical effects of Pinctada maxima nacre chips and the global nacre soluble protein matrix (SPM) on primary human bone marrowderived stromal cells (hBMSCs) in vitro. In static co-culture with nacre chips, the hBMSCs secreted Alkaline phosphatase (ALP) at levels that exceeded bone morphogenetic protein (rhBMP-2) treatment. Concentrated preparation of SPM applied to Stro-1 selected hBMSC’s led to rapid ALP secretions, at concentrations exceeding the untreated controls even in osteogenic conditions. Within 21 days the same population of Stro-1 selected hBMSCs proliferated and secreted collagens I-IV, indicating the premature onset of an osteoblast phenotype. The same SPM was found to promote unselected hBMSC differentiation with osteocalcin detected at 7 days, and proliferation increased at 7 days in a dose-dependent manner. In conclusion, nacre particles and nacre SPM induced the early stages of human bone cell differentiation, indicating that they may be promising soluble factors with osteoinductive capacity in primary human bone cell progenitors such as, hBMSC’s.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Almeida, M. J. / 2001 / Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts / J. Biomed. Mater. Res 57:306~312
Atlan, G. / 1997 / Reconstruction of human maxillary defects with nacre powder : histological evidence for bone regeneration / C. R. Acad. Sci. III 320:253~258
Bédouet, L. / 2006 / Identification of low molecular weight molecules as new components of the nacre organic matrix / Comp. Biochem. Physiol. B Biochem. Mol. Biol 144:532~543
Bédouet, L. / 2007 / Proteomics analysis of the nacre soluble and insoluble proteins from the oyster Pinctada margaritifera / Mar. Biotechnol 9:638~649
Duplat, D. / 2007 / The effect of molecules in mother-of-pearl on the decrease in bone resorption through the inhibition of osteoclast cathepsin K / Biomaterials 28:4769~4778
Atlan, G. / 1997 / Reconstruction of human maxillary defects with nacre powder : histological evidence for bone regeneration / C. R. Acad. Sci. III 320:253~258
Bédouet, L. / 2006 / Identification of low molecular weight molecules as new components of the nacre organic matrix / Comp. Biochem. Physiol. B Biochem. Mol. Biol 144:532~543
Bédouet, L. / 2007 / Proteomics analysis of the nacre soluble and insoluble proteins from the oyster Pinctada margaritifera / Mar. Biotechnol 9:638~649
Duplat, D. / 2007 / The effect of molecules in mother-of-pearl on the decrease in bone resorption through the inhibition of osteoclast cathepsin K / Biomaterials 28:4769~4778
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