구강암종 세포주에서 은행잎 추출물(EGb 761)에 의한 세포고사 기전

장정현; 윤주헌; 최은창; 이근완; 조창일; 김경수

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자료유형: 학술저널

저자정보: 장정현 윤주헌 최은창 이근완 조창일 김경수

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제49권 제12호

발행연도: 2006.1

수록면: 1,181 - 1,187 (7page)

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Background and Objectives：According to our previous study Ginkgo biloba extract (EGb 761) induces inhibition of cell proliferationand apoptosis in SCC 1483 oral cavity cancer cells. On the other hand, in lipopolysaccharide-stimulated RAW 264.7cells, activation of mitogen activated protein kinase (MAPK) is the key event in the inhibition of inflammation by EGb 761. Therefore,we have investigated whether MAPK pathway is involved in the apoptotic process by EGb 761 in oral cavity cancer celllines or not. Subjects and Method：In SCC 1483 oral cavity cancer cell lines, Western blot analysis, Fluorescence-activatedcell sorter (FACS) analysis, and transient transfection using MAPK-dominant negative constructs were used. Results：WhenSCC 1483 oral cavity cancer cell lines were treated with the concentration of 250 μg/ml EGb 761, activation of extracellular signal-regulated kinase (ERK) and apoptosis were noted. This apoptosis was inhibited by the treatment with ERK inhibitor (PD98059). In the transiently transfected cells by MAPK/ERK kinase 1 (MEK1)-dominant negative construct, phosphorylations ofERK and p90 ribosomal S 6 protein kinase (RSK1) were inhibited which led to the inhibition of apoptosis by EGb 761. Theinhibition of apoptosis was also noted in the transfected cells by RSK1 dominant negative construct and cAMP response elementbinding protein (CREB)-dominant negative construct. Conclusion：In conclusion, the apoptosis of SCC 1483 oral cavity cancercell lines by EGb 761 is linked to the activation of ERK and it can happen via ERK MAPK/RSK1/CREB signal transductionpathway. (Korean J Otolaryngol 2006;49:1181-7)

#구강암종·은행잎 추출물·세포고사. #Mouth neoplasms·Ginkgo biloba·Apoptosis.

참고문헌 (17)

참고문헌 신청

/ / State of p53 Rb and DCC tumor suppressor gene in human oral cancer cell lines : 1405 ~ 13

/ / The doyen of trees : 1450 ~ 4

/ 1997 / Role of extracellular signal-regulated protein kinases in apoptosisby asbestos and H2O2 273 : 1029 ~ 1035

/ 1998 / Effect of antioxidants on the functions of cultured rat hepaticstellate cells and Kupffer cells 27 (5) : 1265 ~ 1274

/ 2000 / Drug-induced inhibition of the peripheral-type benzodiazepinereceptor expression and cell proliferation in human breast cancercells 50 (5A) : 2835 ~ 2847

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