지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 환경독성보건학회 Environmental Analysis Health and Toxicology Environmental Health and Toxicology Vol.31
- 발행연도
- 2016.12
- 수록면
- 58 - 65 (8page)
이용수
초록· 키워드
오류제보하기
Objectives Aromatase inhibitors that block estrogen synthesis are a proven first-line hormonal therapy for postmenopausal breast cancer. Although it is known that standardized extract of Ginkgo biloba (EGb761) induces anti-carcinogenic effects like the aromatase inhibitors, the effects of EGb761 on steroidogenesis have not been studied yet. Therefore, the effects of EGb761 on steroidogenesis and aromatase activity was studied using a H295R cell model, which was a good in vitro model to predict effects on human adrenal steroidogenesis.
Methods Cortisol, aldosterone, testosterone, and 17β-estradiol were evaluated in the H295R cells by competitive enzyme-linked immunospecific assay after exposure to EGb761. Real-time polymerase chain reaction were performed to evaluate effects on critical genes in steroid hormone production, specifically cytochrome P450 (CYP11/ 17/19/21) and the hydroxysteroid dehydrogenases (3β-HSD2 and 17β-HSD1/4). Finally, aromatase activities were measured with a tritiated water-release assay and by western blotting analysis.
Results H295R cells exposed to EGb761 (10 and 100 μg/mL) showed a significant decrease in 17β-estradiol and testosterone, but no change in aldosterone or cortisol. Genes (CYP19 and 17β-HSD1) related to the estrogen steroidogenesis were significantly decreased by EGb761. EGb761 treatment of H295R cells resulted in a significant decrease of aromatase activity as measured by the direct and indirect assays. The coding sequence/Exon PII of CYP19 gene transcript and protein level of CYP19 were significantly decreased by EGb761.
Conclusions These results suggest that EGb761 could regulate steroidogenesis-related genes such as CYP19 and 17β-HSD1, and lead to a decrease in 17β-estradiol and testosterone. The present study provides good information on potential therapeutic effects of EGb761 on estrogen dependent breast cancer.
Methods Cortisol, aldosterone, testosterone, and 17β-estradiol were evaluated in the H295R cells by competitive enzyme-linked immunospecific assay after exposure to EGb761. Real-time polymerase chain reaction were performed to evaluate effects on critical genes in steroid hormone production, specifically cytochrome P450 (CYP11/ 17/19/21) and the hydroxysteroid dehydrogenases (3β-HSD2 and 17β-HSD1/4). Finally, aromatase activities were measured with a tritiated water-release assay and by western blotting analysis.
Results H295R cells exposed to EGb761 (10 and 100 μg/mL) showed a significant decrease in 17β-estradiol and testosterone, but no change in aldosterone or cortisol. Genes (CYP19 and 17β-HSD1) related to the estrogen steroidogenesis were significantly decreased by EGb761. EGb761 treatment of H295R cells resulted in a significant decrease of aromatase activity as measured by the direct and indirect assays. The coding sequence/Exon PII of CYP19 gene transcript and protein level of CYP19 were significantly decreased by EGb761.
Conclusions These results suggest that EGb761 could regulate steroidogenesis-related genes such as CYP19 and 17β-HSD1, and lead to a decrease in 17β-estradiol and testosterone. The present study provides good information on potential therapeutic effects of EGb761 on estrogen dependent breast cancer.
목차
Introduction
Materials and Methods
Results
Discussion
References
참고문헌 (38)
참고문헌 신청
정규원 / 2015 / Cancer Statistics in Korea: Incidence, Mortality, Survival, and Prevalence in 2012 / Cancer Research and Treatment 47(2):127~141
Almstrup K / 2002 / Dual effects of phytoestrogens result in u-shaped doseresponse curves / Environ Health Perspect 110(8):743~748
Macedo LF / 2009 / Aromatase inhibitors and breast cancer / Ann N Y Acad Sci 1155:162~173
Mokbel R / 2006 / Adjuvant endocrine therapy for postmenopausal breast cancer in the era of aromatase inhibitors: an update / Int Semin Surg Oncol 3:31
Janni W / 2010 / Adjuvant aromatase inhibitor therapy: outcomes and safety / Cancer Treat Rev 36(3):249~261
Almstrup K / 2002 / Dual effects of phytoestrogens result in u-shaped doseresponse curves / Environ Health Perspect 110(8):743~748
Macedo LF / 2009 / Aromatase inhibitors and breast cancer / Ann N Y Acad Sci 1155:162~173
Mokbel R / 2006 / Adjuvant endocrine therapy for postmenopausal breast cancer in the era of aromatase inhibitors: an update / Int Semin Surg Oncol 3:31
Janni W / 2010 / Adjuvant aromatase inhibitor therapy: outcomes and safety / Cancer Treat Rev 36(3):249~261
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effect of Ginkgo biloba Extract (EGb 761) on Serum Cholesterol Levels in Wild-type C57Bl/6 Mice
대한의생명과학회지
2017 .06
-
Effect of Ginkgo Biloba Extract on N-Methyl-D-Aspartic Acid Receptor Subunit 2B Expression in a Salicylate-Induced Ototoxicity Model
Clinical and Experimental Otorhinolaryngology
2019 .01
-
Analysis of CYP17, CYP19 and CYP1A1 Gene Polymorphisms in Iranian Women with Breast Cancer
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
KRG and its major ginsenosides do not show distinct steroidogenic activities examined by the OECD test guideline 440 and 456 assays
Journal of Ginseng Research
2023 .05
-
11β-Hydroxysteroid Dehydrogenase Type 1 Inhibition Attenuates the Adverse Effects of Glucocorticoids on Dermal Papilla Cells
Yonsei Medical Journal
2017 .01
-
Suicidal gene therapy with rabbit cytochrome P450 4B1/2-aminoanthracene or 4-ipomeanol system in human colon cancer cell
대한방사성의약품학회지
2015 .01
-
The endocrine-related effects of perfluorinated chemicals (PFOA and PFOS) in human carcinoma cell lines (MVLN, 22Rv1 and H295R)
환경독성보건학회 심포지엄 및 학술대회
2015 .06
-
Measurement of Human Cytochrome P450 Enzyme Induction Based on Mesalazine and Mosapride Citrate Treatments Using a Luminescent Assay
Biomolecules & Therapeutics(구 응용약물학회지)
2015 .01
-
Breast Cancer Association with CYP1A2 Activity and Gene Polymorphisms - a Preliminary Case-control Study in Tunisia
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Individualized treatment based on CYP3A5 single-nucleotide polymorphisms with tacrolimus in ulcerative colitis
Intestinal research
2019 .01
-
Sub-acute toxicity and effect of Hwangryunhaedok-tang on human drug-metabolizing enzymes
대한한의학회지
2017 .06
-
Porcine intestinal lymphoid tissues synthesize estradiol
Journal of Veterinary Science
2018 .07
-
Roles of CYP2C9 and its variants (CYP2C9*2 and CYP2C9*3) in the metabolism of 6-methoxy-2-napthylacetic acid, an active metabolite of the prodrug nabumetone
Journal of Pharmaceutical Investigation
2020 .01
-
Gene Expression of CYP1A1 and its Possible Clinical Application in Thyroid Cancer Cases
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Inhibitory Aromatase Effects of Flavonoids from Ginkgo Biloba Extracts on Estrogen Biosynthesis
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
SNP genetic polymorphisms of MDR-1, CYP1A2 and CYPB11 genes in four canine breeds upon toxicological evaluation
Journal of Veterinary Science
2015 .09
-
Association of Cytochrome-17 (MspA1) Gene Polymorphism with Risk of Gall Bladder Stones and Cancer in North India
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Effect of Red Ginseng on cytochrome P450 and P-glycoprotein activities in healthy volunteers
Journal of Ginseng Research
2016 .10
-
Effects of Cellular 11β-hydroxysteroid Dehydrogenase 1 on LPS-induced Inflammatory Responses in Synovial Cell Line, SW982
Immune Network
2017 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2017-539-002198400