구강암종 세포주에서 은행잎 추출물(EGb 761)의 세포고사 효과

이주환; 이광현; 윤주헌; 서형석; 권순호; 김경수

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자료유형: 학술저널

저자정보: 이주환 이광현 윤주헌 서형석 권순호 김경수

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제48권 제3호

발행연도: 2005.1

수록면: 320 - 325 (6page)

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Background and Objectives：To examine the chemopreventive efects of Ginkgo biloba extract (EGb 761) on oral cavity cancer, we investigated the apoptosis of oral cavity cancer cells by EGb 761 and the apoptotic pathway involved. Materials and Method：SCC 1483 cancer cell line derived from a human retromolar trigone carcinoma was used. Inhibition of proliferation was examined by proliferation assay. Apoptosis was measured and confirmed by flow cytometry, DNA fragmentation asay and Western blotting with PARP antibodies. The involvement of the caspase cascade was investigated by co-treating with the caspase inhibitor, z-VAD-fmk. Results：The inhibition of SCC 1483 cells was noted from 250 μg/ml of EGb 761. Apoptosis was observed after 24 hours of incubation with 250 μg/ml EGb 761 and it occured in a time-dependent manner. Apoptosis was confirmed by DNA fragmentation and PARP cleavage. Co-treatment with z-VAD-fmk inhibited apoptosis and PARP cleavage by EGb 761. Conclusions：EGb 761 induces the apoptosis of SC 1483 cells and the caspase cascade is involved in this apoptosis. Therefore, EGb 761 may be used as a chemopreventive agent in oral cavity cancer. Further studies are required on the clinical use of EGb 761.

#은행잎 추출물·구강암·세포고사·Caspase cascade. #Ginkgo biloba· Oral cancer· Apoptosis· Caspases.

참고문헌 (17)

참고문헌 신청

/ 1986 / The doyen of trees:The Ginkgo biloba 15 : 1450 ~ 4

/ 1988 / Preparation and definition of Ginkgo biloba extract Recent results in pharmacologyand clinic : 32 ~ 6

/ 1993 / State of p53,Rb and DCC tumor suppressor genes in human oral cancer cell lines 13 : 1405 ~ 13

/ 1995 / Identification and inhibition of the ICE/CED-3protease necessary for mammalian apoptosis Nature 1995 : 37 ~ 43

/ 1995 / a mammalian homolog of CED-3 is a CrmAinhibitableprotease that cleaves the death substrate poly : 32 801 ~ 9

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