지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 180 - 186 (7page)
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초록· 키워드
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Objective Dysfunction of neural plasticity in the brain is known to alter neural networks, resulting in depression. To understand how fluoxetine regulates molecules involved in neural plasticity, the expression levels of NCAM, NCAM140, CREB and pCREB, in rat C6 glioma cells after fluoxetine treatment were examined.
Methods C6 cells were cultured after 20 min or after 6, 24 or 72 h treatments with 10 μM fluoxetine. Immunocytochemistry was used to determine the effect of fluoxetine on the expression of NCAM. Western blot analysis was used to measure the expression levels of NCAM140 and CREB and the induction of pCREB after fluoxetine treatment.
Results NCAM expression following 72-h fluoxetine treatment was significantly increased around cell membranes compared to control cells. Cells treated with fluoxetine for 6 and 72 h showed a significant increase in NCAM140 expression compared to cells treated for 20 min. The level of pCREB in the cells treated with fluoxetine for 72 h not only increased more than 60%, but was also significantly different when compared with the other treatment times. The 72-h fluoxetine treatment led to the increase of NCAM140 and the phosphorylation of CREB in C6 cells.
ConclusionaaOur findings indicate that fluoxetine treatment regulates neuronal plasticity and neurite outgrowth by phosphorylating and activating CREB via the NCAM140 homophilic interaction-induced activation of the Ras-MAPK pathway.
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참고문헌 (23)
참고문헌 신청
Beggs HE. / 1997 / NCAM140 interacts with the focal adhesion kinase p125(fak) and the SRC-related tyrosine kinase p59(fyn) / J Biol Chem 272 : 8310 ~ 8319
Castren E. / 2005 / Is mood chemistry? / Nat Rev Neurosci 6 : 241 ~ 246
Castren E. / 2010 / The role of BDNF and its receptors in depression and antidepressant drug action: reactivation of developmental plasticity / Dev Neurobiol 70 : 289 ~ 297
Conboy L. / 2009 / The antidepressant agomelatine blocks the adverse effects of stress on memory and enables spatial learning to rapidly increase neural cell adhesion molecul
Cotter D. / 2001 / Reduced glial cell density and neuronal size in the anterior cingulate cortex in major depressive disorder / Arch Gen Psychiatry 58 : 545 ~ 553
Castren E. / 2005 / Is mood chemistry? / Nat Rev Neurosci 6 : 241 ~ 246
Castren E. / 2010 / The role of BDNF and its receptors in depression and antidepressant drug action: reactivation of developmental plasticity / Dev Neurobiol 70 : 289 ~ 297
Conboy L. / 2009 / The antidepressant agomelatine blocks the adverse effects of stress on memory and enables spatial learning to rapidly increase neural cell adhesion molecul
Cotter D. / 2001 / Reduced glial cell density and neuronal size in the anterior cingulate cortex in major depressive disorder / Arch Gen Psychiatry 58 : 545 ~ 553
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