지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 313 - 318 (6page)
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초록· 키워드
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Objective Antidepressants modulate neuronal plasticity. Tianeptine, an atypical antidepressant, might be involved in the restoration of neuronal plasticity; it primarily enhances the synaptic reuptake of serotonin. NCAM140 is involved in neuronal development processes, synaptogenesis and synaptic plasticity. We investigated the effect of tianeptine on the expression of NCAM140 and its downstream signaling molecule in the human neuroblastoma cell line SH-SY5Y.
Methods NCAM protein expression was measured in human neuroblastoma SH-SY5Y cells that were cultivated in serum-free media and treated with 0, 10, or 20 μM tianeptine for 6, 24, or 72 hours. NCAM140 expression in the tianeptine treatment group was confirmed by Western blot, and quantified through measurement of band intensity by absorbance. CREB and pCREB expression was identified after treatment with 20 μM tianeptine for 6, 24, and 72 hours by Western blot.
Results Compared to cells treated for 6 hours, cells treated with 0 or 10 μM tianeptine for 72 hours showed a significant increase in NCAM140 expression and cells treated with 20 μM tianeptine showed a significant increase after 24 and 72 hours. The pCREB level in cells treated with 20 μM tianeptine increased in time-dependent manner.
Conclusion Our findings indicated that the tianeptine antidepressant effect may occur by induction of NCAM140 expression and CREB phosphorylation.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Beracochea D / 1994 / Comparison of the effects of tianeptine and paroxetine on amnesic deficits induced by prolonged alcohol drinking in mice / Encephale 20 : 13 ~ 16
Castren E / 2005 / Is mood chemistry? / Nat Rev Neurosci 6 : 241 ~ 246
Conrad CD / 1996 / Chronic stress impairs rat spatial memory on the Y maze, and this effect is blocked by tianeptine pretreatment / Behav Neurosci 110 : 1321 ~ 1334
Feng Z / 2002 / Neuronal differentiation and protection from nitric oxide-induced apoptosis require c-Jun-dependent expression of NCAM140 / Mol Cell Biol 22 : 5357 ~ 5366
Fujioka T / 2004 / Activation of cAMP signaling facilitates the morphological maturation of newborn neurons in adult hippocampus / J Neurosci 24 : 319 ~ 328
Castren E / 2005 / Is mood chemistry? / Nat Rev Neurosci 6 : 241 ~ 246
Conrad CD / 1996 / Chronic stress impairs rat spatial memory on the Y maze, and this effect is blocked by tianeptine pretreatment / Behav Neurosci 110 : 1321 ~ 1334
Feng Z / 2002 / Neuronal differentiation and protection from nitric oxide-induced apoptosis require c-Jun-dependent expression of NCAM140 / Mol Cell Biol 22 : 5357 ~ 5366
Fujioka T / 2004 / Activation of cAMP signaling facilitates the morphological maturation of newborn neurons in adult hippocampus / J Neurosci 24 : 319 ~ 328
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