비갑개점막의 장기배양(Organ Culture)를 이용한 Rhinovirus 감염의 연구모델

김성학; 권현자; 정유삼; 이봉재; 장용주

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자료유형: 학술저널

저자정보: 김성학 권현자 정유삼 이봉재 장용주

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제47권 제7호

발행연도: 2004.1

수록면: 632 - 638 (7page)

이용수

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연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

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Background and Objectives：To have better understanding on the pathophysiology of rhinovirus infection, an availability of an ideal experimental model is of utmost importance. We aimed to develop a new study model using the organ culture of turbinate mucosa to overcome the limitations of the conventional study methods. Materials and Method：The inferior turbinate mucosae harvested during the septoturbinoplasty were cultured in air-liquid interface methods, placed on the suport of gelfoam soaked in the culture media. Human rhinovirus -16 was applied on the top of the mucosal surface. The success of rhinovirus infection was determined by semi-nested RT-PCR of the mucosal surface fluid taken 48 hours after incubation. Intracellular rhinovirus was SA method. Results：PCR product of 292 bp on semi-nested RT-PCR, representing successful rhinovirus infection, was detected in 5 tissues out of 10 mucosal tissues. In the in situ hybridization method, positively stained cells were found in epithelial layer in scattered fashion. In the analysis of cytokine production by continuous exposure to rhinovirus according to the time course, IL-6 and IL-8 secretions in the infected mucosae were significantly greater than in the control mucosa. The increase in the cytokine production was evident from 24 hours after the infection. Conclusion：The results of this study indicate that the organ culture of turbinate mucosa could serve as an acceptable in vitro model for studying pathophysiology of rhinovirus infection.

#비갑개·장기배양 #Turbinate· Organ culture· Rhinovirus· RT-PCR· In situ hybridization.

참고문헌 (20)

참고문헌 신청

/ 1988 / Kinins are generated during experimentalrhinovirus colds 157 : 133 ~ 142

/ 1990 / Detection of rhinovirus RNA innasal epithelial cells by in situ hybridization 30 : 115 ~ 126

/ 1992 / Domains 1 and 2 of ICAM-1 are sufficient to bind human rhinovirus 24 : 173 ~ 186

/ 1994 / Influence of interleukin-8 challenge in the nasal mucosa in atopicand nonatopic subjects 150 : 1108 ~ 1107

/ 1994 / Interaction of Pseudomonas aeruginosa with human respiratory mucosain vitro 7 : 1746 ~ 1753

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